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高频超声波诱导氧化低密度脂蛋白的制备

Yuanmin Li¹, Wanyue Yang², Xinyi Zhang²

¹Shandong Provincial Hospital Affiliated to Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, China; School of Laboratory Animal& Shandong Laboratory Animal Center, Shandong First Medical University &Shandong Academy of Medical Sciences, Jinan, Shandong 250118, China; Department of Cardiology. The Second Affiliated Hospital of Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, Shandong 271000, China.

Ultrasonics sonochemistry

|March 8, 2025

概括

高频超声波为动脉样硬化研究提供了一种更绿色,更快速的氧化低密度脂蛋白 (LDL) 方法. 超声氧化LDL (U-oxLDL) 在细胞效应方面非常接近氧化铜LDL (Cu-oxLDL).

科学领域:

心血管生物学心血管生物学
生物物理学的生物物理.
生物化学生物化学

背景情况:

泡细胞在动脉样硬化研究中至关重要.
传统的氧化低密度脂蛋白 (oxLDL) 制剂使用铜离子 (Cu2+) 有局限性,包括长氧化时间和残余铜.
开发高效和安全的oxLDL制备方法对于推进动脉样硬化研究至关重要.

研究的目的:

研究高频超声波作为LDL氧化的替代方法.
为了比较超声氧化LDL (U-oxLDL) 与氧化铜LDL (Cu-oxLDL) 和原生LDL (N-LDL) 的特征.
评估U-oxLDL对巨细胞基因表达的影响.

主要方法:

使用高频超声波 (400 kHz) 氧化LDL.
通过电泳流动性,泡细胞形态,脂质含量和胆固醇运输蛋白质对U-oxLDL,Cu-oxLDL和N-LDL的表征.
氧化LDL变体的脂质学分析.
对暴露于oxLDL的骨髓衍生巨细胞 (BMDMs) 的转录组分析.

主要成果:

与Cu-oxLDL相比,U-oxLDL在电泳性移动性,泡细胞形态,脂质含量和胆固醇运输蛋白质方面没有显著差异.
脂质组分析表明U-oxLDL与Cu-oxLDL相比更类似于N-LDL.
用U-oxLDL治疗的BMDM泡细胞中的基因表达模式与用Cu-oxLDL治疗的基因表达模式一致超过90%.

结论:

高频超声波是一种绿色和高效的oxLDL制备方法.
在细胞反应中,U-oxLDL有效地模仿Cu-oxLDL,使其成为动脉样硬化研究的合适替代品.
这种新的方法有望通过提供更可控和潜在的更安全的oxLDL模型来推进动脉样硬化研究.

关键词:

动脉样硬化是一种动脉样硬化.泡细胞是一种泡细胞.高频超声波是一种高频超声波.基基因是基基的组成部分.氧化低密度脂蛋白是一种氧化低密度脂蛋白.

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