相关实验视频

Updated: May 23, 2025

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

埃克1/2-EGR1信号通路参与了小鼠巨细胞中脂聚糖诱导的转化生长因子-β1表达

Weimin Zhao¹, Hong Wang², Chaohui Dai¹

¹Jiangsu Province Engineering Research Center of Precision Animal Breeding, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Microbial pathogenesis

|March 8, 2025

概括

脂多糖 (LPS) 通过Erk1/2-EGR1通路在巨细胞中增强TGF-β1. 这项研究揭示了一种与以前已知的途径截然不同的新机制,增强了我们对免疫反应的理解.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

已知脂聚糖 (LPS) 能刺激转化生长因子β1 (TGF-β1) 的表达.
虽然NF-κB/METTL3/METTL14和AMPK通路有关,但在小鼠巨细胞中,精确的调节机制尚不清楚.

研究的目的:

阐明LPS调节小鼠巨细胞TGF-β1表达的分子机制.
确定参与LPS诱导的TGF-β1表达的关键信号通路和转录因子.

主要方法:

使用RAW264.7小鼠巨细胞系的体外研究.
在LPS挑战后对TGF-β1mRNA和蛋白质水平的分析.
对MAPK (p38,Erk1/2,JNK) 和NF-κB通路激活的研究.
评估早期生长反应1 (EGR1) 的水平和功能.
双 luciferase 报告器和染色体免疫沉-PCR (ChIP-PCR) 试验.

主要成果:

在mRNA和蛋白质水平上,LPS显著增加了TGF-β1的表达.
Erk1/2通路的激活至关重要,因为它的阻断完全取消了LPS诱导的TGF-β1表达.
在LPS治疗中,EGR1水平升高,EGR1是Erk1/2.2的下游调节剂.
抑制EGR1显著降低了LPS诱导的TGF-β1表达.
证实EGR1是关键的转录因子,与TGF-β1促进体结合.

结论:

LPS主要通过Erk1/2-EGR1信号通路调节小鼠巨细胞中的TGF-β1表达.
这种Erk1/2-EGR1通路代表了LPS诱导的TGF-β1表达的新机制,与以前识别的通路不同.

关键词:

在 EGR1 里面,你会看到 EGR1 的位置.这是Erk1/2 在LPS中使用LPS.巨细胞是一个巨细胞.在TGF-β1中.

更多相关视频

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...