从炎症到重塑:一种新的BASP1+单细胞子集作为急性大动脉剖析的催化剂
Wenhui He1, Sanjiu Yu2, Jun Li2
1Department of Biochemistry and Molecular Biology, Army Medical University, Chongqing 400038, China.
Journal of advanced research
|March 8, 2025
概括
脑溶性酸蛋白1阳性 (BASP1+) 单细胞是急性大动脉剖析 (AAD) 发育中的关键免疫细胞. 准这种单细胞子组为早期AAD干预提供了一个新的治疗策略.
科学领域:
- 心血管生物学 心血管生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 单细胞是异质的免疫细胞,在急性大动脉剖析 (AAD) 中的作用尚不清楚.
- 调查单细胞子集对于理解AAD病原体至关重要.
研究的目的:
- 阐明大脑溶性酸蛋白1阳性 (BASP1+) 单细胞子集在AAD中的作用.
- 确定BASP1+单细胞促进AAD的潜在分子机制.
主要方法:
- 人类和小鼠单细胞/巨细胞的单细胞RNA测序 (scRNA-seq).
- 在记者小鼠中的体内血统追踪和单细胞贩运研究.
- 包括Co-IP和ChIP测序在内的分子分析,以调查信号通路.
- 基因操纵 (条件淘汰赛,siRNA) 针对BASP1+单细胞.
主要成果:
- 在AAD中,BASP1+单细胞是早期透者,分化为放大炎症的BASP1+巨细胞.
- 这些单细胞诱导炎症性血管光滑肌细胞 (VSMC) 和富含ROS的内皮细胞 (EC),促进VSMC/EC亡和血管重塑.
- 向BASP1+单细胞或抑制BASP1表达显著降低了小鼠的AAD发育.
结论:
- BASP1+单细胞子集在AAD病变发生过程中起着至关重要的作用.
- 已识别的BASP1+单细胞的调控网络为AAD机制提供了洞察力.
- BASP1+单细胞代表了早期AAD干预的有前途的治疗标.
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