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Updated: Apr 25, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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Published on: February 25, 2010

在Pichia pastoris的多基因代谢工程中,合成了ectoine

Shuai Zhang¹, Bingjie Cheng², Qing Liao²

¹State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Ave., Wuxi 214122, China; School of Biotechnology, Jiangnan University, 1800 Lihu Ave., Wuxi 214122, China; Guangzhou Cn-Ferment Biotechnology Co., Ltd., Guangzhou 510550, China.

Journal of bioscience and bioengineering

|March 8, 2025

概括

皮奇亚牧羊草 (Pichia pastoris) 经过基因工程改造,可以生产生菜,产量为10.88g/L. 这表明它作为一种有效的微生物宿主,可以作为一种有价值的氧化物ectoine生物合成的潜在潜力.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
代谢工程是代谢工程.

背景情况:

乙是用于化品,食品和药品的有价值的溶解物.
之前的ectoine生产依赖于性微生物,并对大肠杆菌,C. glutamicum和H. polymorpha进行了改造.
高密度培养的强壮宿主Pichia pastoris还没有被用于生殖素合成.

研究的目的:

设计Pichia pastoris作为一种新的宿主,用于生殖素的生产.
通过多基因代谢工程来优化生殖素产量.

主要方法:

识别了用于生殖素生产的Chromohalobacter salexigens HZS/E (46.96毫克/毫升).
克隆了ectoine合成酶基因 (ectABC) 并将其引入P. pastoris GS115在不同的促进者 (P_GAP和P_AOX) 下.
通过单独和集体过度表达aspC,aK和asD基因来增强前体供应.

主要成果:

用P_GAP驱动的ectABC (HZS02) 进行工程设计的P. pastoris产生了8.03g/L的ectoine.
用P_AOX驱动的ectABC (HZS01) 进行工程设计的P. pastoris产生了7.13g/L的ectoine.
在P. pastoris中,ectABC与aspC,aK和asD的同时表达实现了10.88g/L的最高产量.

结论:

皮奇亚牧草是一种高度有效的宿主,用于生殖素的生物合成.
代谢工程策略,包括促进者选择和前体路径增强,显著改善了ectoine产量.
这项研究为使用P. pastoris.的工业生态生产开辟了新的途径.

关键词:

埃克托因 (Ectoine) 是一种生态蛋白质.代谢工程是代谢工程.这就是Pichia pastoris.在 ectABC 中.l-酸盐-β-半化物

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