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Updated: May 23, 2025

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用TurboID近距离标记为整个PARP家族的反应器的综合数据集

Jiefu Zheng¹, Yawen Deng^1,2, Cong Fang¹

¹Department of Clinical Laboratory, Shenshan Central Hospital, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Shanwei, 516600, China.

Scientific data

|March 8, 2025

概括

研究人员使用TurboID绘制了PARP家族的蛋白质相互作用,确定了6,314个相互作用体. 这种交互原子图谱揭示了功能关系,并有助于理解PARP生物学和治疗开发.

科学领域:

分子生物学分子生物学
蛋白质组学是指蛋白质组学.
生物化学生物化学

背景情况:

聚基聚合酶 (PARP) 是参与DNA修复和其他细胞过程的关键酶.
了解PARP蛋白相互作用是阐明它们多样化的功能的关键.
现有的方法往往无法捕捉短暂或低丰度的蛋白质相互作用.

研究的目的:

为PARP蛋白家族生成一个全面的互动组图.
为了确定各种PARP成员的新型蛋白质相互作用体,包括短暂的蛋白质相互作用体.
为研究界提供公开可访问的资源.

主要方法:

使用 TurboID 近距离标记与 V5 标记的融合结构进行体内蛋白质标记.
采用质谱法来识别和量化标记的相互作用蛋白质.
进行GFP-PARP本地化实验以验证亚细胞分布.
进行了网络和功能注释分析.

主要成果:

鉴定了6314种高可信度的PARP相互作用蛋白.
捕获了传统技术遗漏的过渡性蛋白质相互作用.
在不同的PARP家族成员中揭示了共享和独特的交互器.
网络分析表明,PARP成员之间存在功能合作和专业化.

结论:

生成的PARP互动原子图谱为研究PARP介导的调节机制提供了基础资源.
这些发现支持PARP家族内的功能专业化和合作性.
这一数据集将促进对PARP生物学的进一步研究,并有助于治疗开发.

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