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维-ATPase有助于Candida albicans-Streptococcus mutans生物膜的空性

Qian Jiang^1,2,3, Mengmeng Xu^1,4, Hong Chen^1,4

¹Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases, 404100, Chongqing, China.

NPJ biofilms and microbiomes

|March 8, 2025

概括

在Candida albicans中准真空质子 (V-ATPase) 可以预防牙损伤. 在双物种模型中,破坏V-ATPase基因 (VMA3,VMA4,VMA11) 会减少生物膜的形成和的严重程度.

科学领域:

微生物学微生物学
口腔健康口腔健康
生物化学生物化学

背景情况:

坎迪达阿尔比坎斯 (Candida albicans) 和突变性链球菌 (Streptococcus mutans) 之间的相互作用对牙虫的发展至关重要.
空腔质子 (V-ATPase) 调节了C. albicans的生长和毒性,成为预防虫的潜在目标.
需要进一步研究V-ATPase在C. albicans-S. mutans生物膜的空性中的作用.

研究的目的:

为了研究V-ATPase对C. albicans-S. mutans生物膜的 cariogenicity的影响.
探索V-ATPase作为牙损伤预防策略的目标的潜力.

主要方法:

检测C.albicans在脏活跃和无脏儿童中的检测.
在C. albicans.中对V-ATPase编码基因 (VMA3,VMA4,VMA11) 的基因淘汰.
在双种生物膜中评估生物膜生物质量,细胞外多糖 (EPS) 生产和生物膜结构.
转录组分析以了解V-ATPase的代谢影响.
在体内评估的严重程度,斑块生物质和EPS的产生.

主要成果:

在脏活跃的儿童中,C. albicans检测和V-ATPase基因表达更高.
V-ATPase基因淘汰突变体表现出减少的生长,抑制的状细胞形成和细胞壁完整性的损害.
由V-ATPase突变体形成的双重生物膜显示生物质显著减少,EPS生产和结构改变.
转录组分析揭示了V-ATPase在C. albicans代谢和S. mutansEPS调节中的作用.
在体内研究表明,在V-ATPase基因被删除后,的严重程度,斑块生物量和EPS的产生减少.

结论:

维-ATPase显著调节了C. albicans-S. mutans生物膜的空性.
向V-ATPase,特别是VMA3,VMA4和VMA11基因,为预防牙提供了一个有前途的策略.
这项研究为开发新的生态方法来管理牙提供了机制基础.

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