发育期暴露于空气污染会增加呼吸道过敏反应,改变转录组和DNA甲基化在女性成年后代
Razia Zakarya1,2, Yik Lung Chan3,4, Baoming Wang3,4
1School of Life Sciences, University of Technology Sydney, Sydney, Australia. Razia.Zakarya@uts.edu.au.
Communications biology
|March 8, 2025
概括
母亲暴露于细颗粒物 (PM2.5) 空气污染对子女喘有表观遗传影响. 暴露母亲的后代表现出基因表达的减少和肺部DNA甲基化的改变,增加了喘的严重程度.
科学领域:
- 环境表观遗传学环境表观遗传学
- 呼吸系统医学 呼吸系统医学
- 毒理学 毒理学 毒理学
背景情况:
- 母亲暴露于微粒空气污染 (PM2.5) 与子女喘发病率和严重程度的增加有关.
- 潜在的机制,特别是表观遗传修饰,在很大程度上仍然不清楚.
- 遗传易感点 (loci) 仅解释了这种观察到的效应的一小部分.
研究的目的:
- 调查母亲暴露于空气污染和后代喘发展之间的表观遗传关联.
- 评估母体PM2.5暴露对后代过敏呼吸道疾病中的转录组和甲基组特征的影响.
- 阐明表观遗传学在调解产前暴露于空气污染的后代中喘严重程度增加中的作用.
主要方法:
- 利用过敏呼吸道疾病的小鼠模型来模拟喘的发展.
- 孕妇暴露在缩的环境PM2.5.5中.
- 分析后代肺组织的转录变化 (基因表达) 和整个基因组的CpG甲基因组变化后的过敏原挑战.
主要成果:
- 母亲的PM2.5暴露显著增加了成年后代的过敏呼吸道疾病严重程度.
- 暴露于母体空气污染的后代表现出明显抑制的对过敏原挑战的转录组反应,与对照组相比,差异表达基因减少了80%.
- 全基因组甲基化分析显示,暴露母亲的后代的差异甲基化区域显著减少,特别是在内部区域和某些可转移元素中.
结论:
- 母亲暴露于PM2.5会导致后代的表观遗传变化,特别是减少基因表达和肺部DNA甲基化模式的变化.
- 这些表观遗传修饰与喘严重程度的增加和成年后代对过敏原的转录基因反应的减弱有关.
- 这项研究提供了证据,表明母亲暴露于空气污染对后代喘的发展和严重性的显著表观遗传贡献.
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