相关实验视频

Updated: May 23, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

微RNA-140-3p通过向肝细胞生长因子,在急性原核细胞白血病中起到瘤抑制基因的作用

RuiQi Lei¹, Xin Liu¹, YiTing Wu¹

¹Division of Hematology, Department of Medicine, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nan Gang District, Harbin, Heilongjiang Province, 150001, China.

Cell biochemistry and biophysics

|March 8, 2025

概括

微RNAs (miRNAs) 调节基因表达,在急性肌肉细胞白血病 (APL) 中至关重要. 抑制 miR-140-3p 抑制APL细胞增殖并促进分化,提供治疗潜力.

科学领域:

分子生物学分子生物学
血液学血液学血液学
在瘤学瘤学.

背景情况:

微RNAs (miRNAs) 是基因表达的关键调节者.
miRNAs在造血干细胞分化中起着重要作用,并与急性肌肉细胞白血病 (APL) 有关.
在复发APL中观察到不同的miRNA表达,miR-140-3p显著下调.

研究的目的:

研究miR-140-3p在APL中的作用.
为了识别miR-140-3p.p.的直接向基因.
在APL中探索调节miR-140-3p的治疗潜力.

主要方法:

双 luciferase 试验以确认直接准.
在NB4细胞的lentiviral转化中操纵miR-140-3p表达.
流细胞计和增殖试验用于评估细胞行为.

主要成果:

肝细胞生长因子 (HGF) 被确定为miR-140-3p的直接向基因.
低miR-140-3p表达抑制了NB4细胞分化和细胞亡,同时促进了细胞增殖和细胞周期进展.
抑制miR-140-3p的升级抑制了增殖,阻止了细胞循环,并增强了APL细胞的亡和分化.

结论:

高杆菌是miR-140-3p的直接目标.
在APL细胞中,miR-140-3p上调具有抗增殖和促分化的作用.
监测miR-140-3p和HGF水平可以预测APL复发风险,并且针对miR-140-3p/HGF途径显示了治疗复发APL的前景.

关键词:

患有急性促细胞性白血病.在HGF的基础上.在MiR-140-3p中使用.微RNAs是一种微RNA.复发情况复发情况

更多相关视频

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...