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有关阿皮乌姆 (Apium graveolens) 的阿斯伯吉卢斯 (Aspergillus sp. : 代谢概况和抗MRSA潜力得到了in silico研究的支持

Alshymaa Abdel-Rahman Gomaa¹, Hesham A Abou-Zied², Sara Mahmoud Farhan³

¹Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.

Microbial cell factories

|March 8, 2025

概括

这项研究发现,从内菌中提取的提取物Aspergillus sp. SH1显示出对抗抗甲素耐药黄金葡萄球菌 (MRSA) 的强有力的活性. 这种真菌提取物可能会导致对抗MRSA感染的新疗法.

科学领域:

微生物学微生物学
自然产品化学自然产品化学
药理学药理学是指药理学的学科.

背景情况:

甲素耐药黄金葡萄球菌 (MRSA) 是难以治疗的医疗相关感染的主要原因.
抗生素耐药性的出现需要发现新的治疗剂.
内生菌被认为是具有抗菌性质的生物活性化合物的有前途来源.

研究的目的:

为了研究内性真菌Aspergillus sp.的抗MRSA潜力. 这就是SH1.
进行真菌提取物的代谢分析,以确定生物活性化合物.
评估已识别的化合物与MRSA相关的标的in silico结合相互作用.

主要方法:

液体染色学-高分辨率电子喷雾电离质谱仪 (LC-HR-ESI-MS) 用于代谢分析.
在体外抗MRSA活性测定以确定IC50值.
在基分子对接研究中,针对MRSA标 (TLR2,VWF,TNF,PBP2a).

主要成果:

代谢分析发现了来自Aspergillus sp.的27种化合物. 一个SH1提取物.
这种真菌提取物表现出强烈的抗MRSA活性,IC50为9.8μg/mL,性能优于西普洛素.
几种化合物 (2,9,15,16,20,22,25) 在中显示出与MRSA标具有显著的结合 afinities.

结论:

亚斯伯菌 sp. 的情况. SH1提取物对MRSA具有显著的抗菌活性.
这些已识别的化合物显示出开发新型抗MRSA药物的分子的潜力.
这项研究支持探索内菌作为对抗抗生素耐药细菌的来源.

关键词:

阿皮亚木 (Apium graveolens) 是一个植物.亚斯珀吉洛斯 (Aspergillus) 是一种植物.这是一种抗MRSA的药物.在状的中.

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