多能性的编程和生殖系从一个Nanog祖先共同进化
Darren Crowley1, Luke Simpson1, Jodie Chatfield1
1School of Life Sciences, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK.
Cell reports
|March 9, 2025
概括
研究人员发现了一种古老的基因Vent,该基因可以编程细胞多能性和生殖线能力. 这一发现揭示了脊椎动物及其祖先关键发育过程的进化起源.
科学领域:
- 发展生物学 发展生物学
- 进化生物学 进化生物学
- 遗传学 遗传学 是一个
背景情况:
- 进化新性往往来自于对现有的遗传机制的修补.
- 脊椎动物的进化依赖于多能性,即细胞形成胚胎层和胚胎细胞 (PGC) 的能力.
- 多能性的起源尚不清楚,因为像Nanog这样的关键调节剂在脊椎动物之外没有保存.
研究的目的:
- 为了调查祖先的无脊椎动物基因是否具有类似纳诺基的活性.
- 了解多能性和生殖系能力调节者的进化历史.
主要方法:
- 测试了来自各种物种的Vent基因 (甲状腺动物,甲状腺动物,海绵动物,脊椎动物) 对纳米动物的活性.
- 使用纳米缺陷小鼠细胞 (iPSCs) 和轴突胚胎进行功能测试.
- 进行了VENTX的敲除,以评估其在生殖线发育中的作用.
主要成果:
- 半弦管 (Saccoglossus kowalevskii) 在小鼠 iPSC 和获救的 Nanog-贫乏的轴突胚胎中编程了多能性.
- 尼达利亚风口 (Nematostella vectensis) 显示部分活动,而海绵和脊椎动物风口没有活动.
- 萨科格洛斯萨斯·文特 (Saccoglossus Vent) 拯救了轴突中的生殖线竞争性中皮质缺陷,与尼马托斯特拉·文特 (Nematostella Vent) 不同.
结论:
- 最后一个双胞胎体祖先的Vent基因可能具有多能性编程和生殖线能力功能.
- 这表明脊椎动物进化之前的关键发育调节者的古代起源.
- 识别了Vent作为一个潜在的祖先纳米类因素,对早期动物发育至关重要.
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