对聚烯微塑料的免疫反应:通过斑马鱼 (Danio rerio) 的ROS驱动的NF-κB通路调节炎症反应
Jincheng Pei1, Shannan Chen2, Qingxia Ke3
1Hubei Key Laboratory of Multi-media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science & Engineering, Huazhong University of Science and Technology (HUST), Wuhan, Hubei Province 430074, PR China.
Aquatic toxicology (Amsterdam, Netherlands)
|March 9, 2025
概括
水生环境中的微塑料会对鱼类造成伤害. 这项研究表明,聚烯MPs通过诱导氧化应激和激活NF-κB信号通路来触发斑马鱼的炎症.
科学领域:
- 环境毒理学环境毒理学
- 水生生态毒理学 水生生态毒理学
- 免疫毒理学 免疫毒理学
背景情况:
- 微塑料 (MP) 污染在水生生态系统中越来越令人担忧.
- 鱼类中MPs的确切免疫毒理学机制尚不清楚.
- 了解MP的毒性对于评估对水生生物的风险至关重要.
研究的目的:
- 为了研究聚烯MPs (PS-MPs) 在斑马鱼中的免疫毒理学影响.
- 为了阐明PS-MP诱导的炎症的潜在机制.
- 探索氧化应激在MP毒性中的作用.
主要方法:
- 使用斑马鱼幼虫和斑马鱼胚胎纤维细胞 (ZF4) 作为模型.
- 通过摄入给斑马鱼幼虫的3微米PS-MPs.
- 评估行为变化,组织病理学,基因表达,氧化应激标志物 (ROS,MDA),抗氧化酶活性和白细胞招募.
- 进行细胞测试以评估ZF4细胞中的细胞内ROS和NF-κB P65转位.
主要成果:
- 斑马鱼幼虫在肠道中积累了PS-MPs,表现出改变的行为和病理学变化.
- 暴露于PS-MPs引发了氧化应激和炎症,由上调的OS/炎症基因,增加的ROS/MDA,改变的抗氧化酶活性和白细胞招募证明.
- 在ZF4细胞中,PS-MPs提高了细胞内ROS和增强了NF-κB P65核转位.
- 抑制ROS缓解NF-κB激活和炎症性细胞因子水平.
结论:
- 聚烯MPs通过ROS触发的NF-κB信号通路诱导斑马鱼的免疫毒性.
- 氧化应激是鱼类PS-MP诱导的炎症的关键媒介.
- 这项研究提供了关于鱼类对微塑料暴露的先天免疫反应的见解.
相关概念视频
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:28Inflammatory Response
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...