一氧化碳抑制了由SARS-CoV-2尖端RBD蛋白诱导的人类支气管上皮的CCL5和IL-6分泌物
Xiao-Min Fang1, Xing-Jian Liu1, Rui-Gang Zhang1
1Department of Physiology, Basic Medical School, Guangdong Medical University, Zhanjiang, China.
Experimental cell research
|March 9, 2025
概括
一氧化碳 (CO) 对人类支气管细胞具有抗炎作用. 它抑制了SARS-CoV-2尖峰RBD诱导的细胞因子释放和ERK酸化,减少了炎症.
科学领域:
- * 呼吸系统医学 呼吸系统医学
- * 分子生物学 * 分子生物学
- * 免疫学 免疫学
背景情况:
- *一氧化碳 (CO) 以其抗炎性质而闻名.
- *CO对人类支气管上皮细胞中SARS-CoV-2尖端受体结合域 (S-RBD) 诱导的炎症反应的影响尚未完全理解.
- *了解这些相互作用对于开发针对病毒性呼吸道感染的治疗策略至关重要.
研究的目的:
- * 为了研究CO对SARS-CoV-2S-RBD诱导的人类支气管上皮细胞中细胞因子释放的抗炎作用.
- *确定CO在调节与炎症有关的细胞外核酸信号通路中的作用.
- * 阐明CO对S-RBD诱导的炎症的保护作用的分子机制.
主要方法:
- *人类支气管上皮细胞 (16HBE14o-) 用S-RBD和CO释放分子3 (CORM-3) 进行治疗.
- * 细胞因子分泌 (CCL5,IL-6) 用酶相关免疫吸收试验 (ELISA) 来测量.
- *ERK酸化通过西部斑分析进行评估,并使用短路电流 (ISC) 量化离子运输.
主要成果:
- * S-RBD显著诱导了CCL5和IL-6的释放,而这种释放被CO抑制.
- *CO没有抑制S-RBD诱导的ATP释放,但降低了ATP诱导的细胞因子分泌和离子运输.
- *CO明显抑制了S-RBD诱导的ERK酸化.
结论:
- * CO在被S-RBD和细胞外核酸刺激的人类支气管上皮细胞中表现出显著的抗炎作用.
- *CO调节炎症信号通路,包括ERK酸化和细胞外核酸反应.
- *这些发现突显了CO作为治疗S-RBD相关呼吸道炎症的治疗剂的潜力.
更多相关视频
09:02Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
Published on: September 22, 2023
1.1K
07:36Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
Published on: February 28, 2021
2.8K
相关概念视频
Chronic Obstructive Pulmonary Disease-II: Pathophysiology
2.7K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
2.7K
Chronic Obstructive Pulmonary Disease
1.1K
COPD is defined as a heterogeneous lung condition marked by persistent respiratory symptoms such as dyspnea, cough, and sputum production, caused by abnormalities in the airways that cause airflow obstruction.
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
1.1K
Breathing
58.1K
The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...
58.1K
