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流通的微RNA与尿相关:中国的一项基于社区的多中心研究

Wending Li¹, Zhaoyang Li², Jixuan Ma²

¹Department of Occupational and Environmental Health, Key Laboratory of Environment and Health, Ministry of Education and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, 10032, USA.

Environmental research

|March 9, 2025

概括

这项研究确定了七种与中国人口暴露相关的血微RNA (miRNA). 这些miRNA可以作为毒性和癌症风险的生物标志物.

科学领域:

环境健康环境健康
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
毒理学毒理学毒理学

背景情况:

暴露是与包括癌症在内的各种疾病相关的全球健康问题.
血微RNAs (miRNAs) 作为暴露的生物标志物的作用尚不清楚.
表观遗传修饰,如miRNA改变,与诱导的毒性有关.

研究的目的:

在中国人群中识别与暴露相关的血微RNA (miRNA).
研究这些miRNAs作为毒性和致癌性的生物标志物的潜力.
阐明对健康影响的表观遗传机制.

主要方法:

一个两阶段的研究设计,涉及发现 (希门小组) 和验证 (武汉-珠海小组) 队列.
使用微阵列和测序进行血miRNA分析.
通过感应联等离子体质谱法量化尿液和血中的含量.

主要成果:

在发现阶段,16个miRNA在高尿和低尿组之间被差异表达.
在验证阶段,七种miRNAs (miR-101-3p,miR-142-3p,miR-148a-3p,miR-15a-5p,miR-199a-3p,miR-27b-3p,miR-340-5p) 与尿液中含量呈正相关性.
其中5种经过验证的miRNA也与血含量有关,并且与癌症相关的途径有关.

结论:

特定的血miRNAs是评估暴露的有希望的生物标志物.
这些已识别的miRNA为引发的毒性和癌症的表观遗传机制提供了洞察力.
进一步的研究可以探索针对这些miRNA途径的治疗策略.

关键词:

亚是一种.与癌症相关的途径循环中的微RNAs.这是一项多中心研究.两个阶段的设计设计.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...