治疗性X射线暴露对原I和相关蛋白质的影响
Ren Jie Tuieng1, Catherine Disney2, Sarah H Cartmell3
1Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester M13 9PT, UK; Singapore Nuclear Research and Safety Initiative, National University of Singapore, Singapore 118415.
Acta biomaterialia
|March 9, 2025
概括
在放射治疗中使用的X射线可以损害细胞外基质 (ECM),包括原I,即使在低剂量. 这种对ECM蛋白质的结构损伤可能会导致细胞损伤以外的放射治疗副作用.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 放射学 放射学是一门学科.
背景情况:
- 生物组织在医学成像 (微型CT) 和治疗 (放射治疗) 中遇到X射线.
- 细胞对X射线的反应是可以理解的,但X射线对细胞外基质 (ECM) 的影响是不能理解的.
- 放射治疗剂量可以达到50Gy,由于细胞损伤导致副作用.
研究的目的:
- 研究X射线诱导的ECM蛋白质和ECM丰富的组织的结构损伤.
- 为了测试原I和老鼠尾部肌是否因放射治疗X射线剂量而受到结构损害.
主要方法:
- 蛋白质凝电泳以分析 I 原蛋白碎片化.
- 液体染色学-双重质谱学 (LC-MS/MS) 和位指纹 (PLF) 用于描述结构变化.
- 纯化原I和肌组织暴露于不同剂量的X射线 (放射治疗和同步射线).
主要成果:
- 放射治疗相当的X射线剂量碎片化溶解的原I.
- 纤维状原I抵抗了碎片化,但显示了结构变化.
- 高剂量的同步X射线导致溶解和纤维状原体I的显著碎片化.
- 同步照射改变了肌中的多种ECM蛋白质的结构,包括原蛋白,蛋白质糖和纤维素-1.
结论:
- 放射治疗X射线可以结构性地改变关键的ECM组件,如原I.
- 对ECM的损伤可能导致放射治疗的副作用.
- 需要进一步的研究来了解剂量依赖于ECM结构的影响.
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