Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.7K
Regulated Protein Degradation02:58

Regulated Protein Degradation

7.1K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.1K
The Proteasome01:13

The Proteasome

790
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
790
The Proteasome Structure01:17

The Proteasome Structure

673
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
673

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Herpes B virus and the neglected risk of zoonotic spillover.

One health (Amsterdam, Netherlands)·2026
Same author

Circulating Amino Acid Network Remodeling Reveals Systemic Metabolic Reprogramming Predictive of Colorectal Cancer Recurrence and Metastasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A Co-opted Developmental Gene Regulatory Program in Endothelial Progenitors Promotes Tumor Angiogenic Phenotypes.

Cancer research·2026
Same author

A Super-Adhesive Air Filter With Capillarity-Mediated Spontaneous Particle Absorption via Dynamic Bond Exchange.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Glutaredoxin-1 attenuates transactive response DNA-binding protein 43-induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation.

Neuroreport·2026
Same author

Therapeutic targeting of blood-derived protein infiltration to modulate neuroinflammation in cerebellar ataxia.

Journal of neuroinflammation·2026
Same journal

Epigallocatechin gallate suppresses adipogenesis by targeting tetranectin and inhibiting its endocytosis via modulation of ERK and AKT signaling pathways.

BMB reports·2026
Same journal

Immunoproteasome inhibition protects the retina from Alzheimer's-associated degeneration.

BMB reports·2026
Same journal

Urolithin A improves mitochondrial dysfunction induced by oxidative stress in human dermal papilla cells.

BMB reports·2026
Same journal

Whole genome-based polygenic risk score and structural variation analysis in Korean patients with inflammatory bowel disease.

BMB reports·2026
Same journal

EK-16005, a 2-anilinopyrimidine derivative, inhibits breast cancer progression by regulating myeloid-derived suppressor cells.

BMB reports·2026
Same journal

Immune phenotype-based stratification of colorectal cancer reveals subtype-specific immunotherapeutic opportunities: insights from a Korean patient cohort.

BMB reports·2026
查看所有相关文章

相关实验视频

Updated: May 23, 2025

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

214

脱基因酶动态:理解基质相互作用的方法.

Sang-Ah Park, Ji Min Lee1

  • 1Graduate School of Medical Science & Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.

BMB reports
|March 9, 2025
PubMed
概括
此摘要是机器生成的。

研究二维基因酶 (DUB) 和它们的点是理解蛋白质平衡的关键. 本综述涵盖了研究DUB基质相互作用的方法,有助于治疗开发.

更多相关视频

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

390
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

相关实验视频

Last Updated: May 23, 2025

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

214
Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

390
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 脱基酶 (DUBs) 是关键的酶,通过去除泛素链来调节蛋白质稳态.
  • 了解DUB-基质相互作用对于破译细胞信号,蛋白质稳定性和降解途径至关重要.
  • 阐明这些相互作用具有重要的治疗潜力.

研究的目的:

  • 审查和突出关键的方法,以调查duebiquitinase (DUB) 的活动和基质相互作用.
  • 为各种实验方法的优点和局限性提供见解.
  • 为更深入的理解,强调整合互补方法的重要性.

主要方法:

  • 生物化学分析:蛋白质降解率,无处不在的动态,蛋白质与蛋白质的相互作用.
  • 基于光的技术:实时监测活细胞中的DUB动态和基质周转 (例如,可光转换的报道器,光计时器,FRET).
  • 试验室二化试验:对DUB在特定基质上的活性有直接的机理洞察.

主要成果:

  • 生物化学方法提供了对DUB功能和特异性的洞察.
  • 光方法可以在活细胞中监测DUB动态.
  • 试管测试提供了关于DUB与基质相互作用的直接机械学数据.
  • 每种方法都有局限性,包括特异性,敏感性,技术挑战或生理相关性.

结论:

  • 整合多种方法提高了准确性,并提供了对DUB-基板相互作用的全面见解.
  • 综合方法对于推动开发针对DUB的治疗策略至关重要.
  • 结合这些技术的进一步研究将促进新的治疗干预措施.