相关实验视频

Updated: May 23, 2025

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Published on: December 16, 2022

通过矩阵辅助激光脱/离子化质谱成像,可视化番茄果中的代谢物在不同成熟度阶段的空间分布

Sihui Guan¹, Chaochao Liu², Zhuping Yao³

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Vegetable Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; College of Agriculture, Shihezi University, Shihezi 832003, China.

Food research international (Ottawa, Ont.)

|March 9, 2025

概括

番茄果实成熟过程涉及代谢物水平和空间分布的显著变化. 糖和挥发性有机化合物 (VOCs) 等关键化合物在果皮附近集中,因为水果成熟到红色.

科学领域:

植物生理学植物生理学
农业科学农业科学
代谢学代谢学代谢学

背景情况:

番茄是全球重要的作物,由于其微量营养素含量,对人类营养至关重要.
了解番茄果实成熟期间代谢物的空间分布对于评估营养和功能价值至关重要.
目前关于番茄果代谢物在成熟过程中的空间动态的知识有限.

研究的目的:

想象和分析番茄果实内源代谢物在四个不同的成熟阶段 (从绿色到红色) 的空间分布.
调查番茄果实发育过程中代谢物度和位置如何变化.
提供有关番茄中植物化学物质生理作用和营养价值的见解.

主要方法:

使用矩阵辅助激光脱/电离质谱成像 (MALDI-MSI) 来可视化代谢物分布.
在四个成熟阶段分析了番茄果实,从绿色到红色.
对各种代谢物进行了相对量化和空间分布成像,包括糖,氨基酸,有机酸和挥发性有机化合物 (VOC).

主要成果:

可溶性糖,氨基酸和挥发性有机化合物 (VOC) 在红色成熟阶段显著增加.
酸水平下降,而酸水平显示初始下降,然后在成熟过程中增加.
可溶性糖,有机酸和氨基酸均分布,而9种VOC从扩散分布转移到成熟水果的皮附近的局部化.

结论:

在番茄果实成熟期间,代谢物概况和空间分布发生了显著的变化.
芳香化合物 (VOCs) 随着水果的成熟而集中在水果的外部区域.
这些发现为番茄育种,作物利用和生产策略提供了科学基础,通过详细说明植物化学动力学.

关键词:

果实果实是一种水果.马尔迪-MSI是什么意思代谢物的代谢物茄子番茄子番茄子挥发性有机化合物挥发性有机化合物

更多相关视频

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

相关概念视频

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...