基于西方饮食的NutriCol介质:一种高pectin,低inulin培养介质在PolyFermS和M-ARCOL连续体外模型中促进了肠道微生物群的稳定性和多样性
Galal Ali Esmail1, Ophélie Uriot2, Walid Mottawea3
1NuGut Research Platform, School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Food research international (Ottawa, Ont.)
|March 9, 2025
概括
一种新的发酵介质,营养性结肠 (NutriCol),在体外结肠模型中比麦克法兰介质更好地支持肠道微生物群的多样性和结构. NutriCol增强了微生物多样性和短链脂肪酸 (SCFA) 生产,提供了更准确的模拟人类结肠环境.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 生物技术是生物技术.
背景情况:
- 精确模拟体外人类结肠环境对于研究肠道微生物群和开发有针对性的干预措施至关重要.
- 现有的发酵介质,如麦克法兰,可能不会精确地反映人类结肠的复杂菌成分和纤维含量.
- 开发优化的介质对于提高体外结肠模型的可靠性和适用性至关重要.
研究的目的:
- 开发和评估一种新的发酵介质,NutriCol,旨在模仿西方饮食中的纤维含量.
- 使用两种不同的体外结肠模型 (PolyFermS和M-ARCOL) 对比NutriCol与已建立的MacFarlane介质的性能.
- 评估NutriCol对肠道微生物群多样性,社区结构,短链脂肪酸 (SCFA) 生产和气体产生的影响.
主要方法:
- 营养Col介质的配方是为了复制典型的西方饮食中的纤维成分,其中含有特定数量的土豆粉,pectin,xylan,arabinogalactan,guar gum,glucomannan和inulin.
- 在两种体外结肠模型中评估了NutriCol的性能:PolyFermS (近接结肠模拟) 和M-ARCOL (光膜和粘膜模拟).
- 微生物群的α-多样性,社区结构 (布雷-柯蒂斯距离,PERMANOVA),微生物丰富度 (ASV数量),SCFA生产和肠道气体水平被分析并与NutriCol和MacFarlane媒体进行了比较.
主要成果:
- 与MacFarlane介质相比,NutriCol在PolyFermS模型中保持了较高的肠道微生物群α多样性.
- 在PolyFermS和M-ARCOL模型中,在两种介质之间观察到微生物群落结构的显著差异.
- 在M-ARCOL模型中,NutriCol显示SCFA (乙酸盐,丁酸盐) 生产增加和肠道气体产生减少,这归因于特定的SCFA生产细菌的丰富.
结论:
- 与MacFarlane相比,NutriCol是一种优越的发酵介质,可以支持肠道微生物群的多样性和结构,特别是在模拟近端结肠时.
- 努特里科尔的疗效取决于特定的体外结肠模型和捐赠者的微生物组合.
- 努特里科尔为体外结肠研究提供了更准确,更有效的工具,增强SCFA生产并降低特定模型中的气体水平.
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