相关实验视频

Updated: May 23, 2025

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

在Pichia pastoris中,从多种碳来源可持续地生产利的代谢工程

Xiaocong Lu^1,2, Mingxin Chang^1,2, Xiangyu Li¹

¹State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Microbial cell factories

|March 10, 2025

概括

这项研究使用工程微生物增强了糖的产量,从葡萄糖中获得了创纪录的产量. 像甘和甲醇这样的可持续原料也显示出对环保利醇制造的承诺.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
工业微生物学工业微生物学

背景情况:

利醇是食品和制药中的一种有价值的化合物.
传统的西利生产对于大规模应用来说是不可持续的.
需要使用替代的,环保的生产方法.

研究的目的:

通过微生物细胞工厂来增强利醇生产.
为了优化新陈代谢途径以增加利醇产量.
探索微生物利醇合成的可持续原料.

主要方法:

微生物细胞工厂的代谢工程.
两个合成途径的组合.
介绍了一种新的NADPH依赖性西利脱酶.
使用可持续原料,如糖和甲醇.

主要成果:

使用微生物系统实现了0.14克西利托尔/克葡萄糖的创纪录的高产量.
从糖醇制造出7000mg/L的糖醇,其产量为0.35g的糖醇/g的糖醇.
从甲醇中制造出250mg/L的氧化醇.
通过代谢工程证明了通过代谢工程显著增强西醇生产.

结论:

微生物细胞工厂的代谢工程为可持续的利醇生产提供了一个有希望的途径.
优化的途径和新型酶显著提高了西利的产量.
像甘和甲醇这样的可持续原料对于具有成本效益的利醇制造是可行的.
这项研究为工业规模的生物技术西利生产提供了基础.

关键词:

这就是Pichia pastoris.代谢工程是代谢工程.可持续的碳来源可持续的碳来源克西利托尔生物合成

更多相关视频

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...