相关实验视频

Updated: May 23, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

基于原始纸的免疫测试装置,使用CRISPR/Cas12a信号放大

Hikaru Suzuki¹, Guodong Tong¹, Pabitra Nath²

¹Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.

|March 10, 2025

概括

这项研究将CRISPR/Cas12a与纸质ELISA集成,用于快速,敏感的蛋白质检测. 这种新方法显著提高了乙型肝炎表面抗原等点的检测极限,使得点诊断成为可能.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
测试开发测试开发

背景情况:

传统的酶相关免疫吸收试验 (ELISA) 对于临床诊断是耗时的.
纸质ELISA提供更快,更便宜的分析,但往往缺乏灵敏度.
纸质测试的有限灵敏度阻碍了对低度标的临床应用.

研究的目的:

为非核酸目标开发一种简单,快速和高度灵敏的检测方法.
将CRISPR/Cas12a系统集成到纸质ELISA平台中.
为了提高基于纸张的免疫试验的敏感性,用于临床诊断.

主要方法:

设计了一种基于纸张的原木类型装置用于测试操作.
使用CRISPR/Cas12a酶与光体/灭器标记的ssDNA探针进行光检测.
采用用DNA标记的抗体来激活CRISPR/Cas12a以提高信号放大.

主要成果:

对人类IgG和B型肝炎病毒表面抗原 (HBsAg) 的检测灵敏度显著提高.
与商业ELISA套件 (200 pg/mL) 相比,HBsAg的检测极限 (LOD) 为12 pg/mL,比商业ELISA套件提高了10倍以上.
在使用智能手机成像进行定量分析的猪全血样本中验证光检测.

结论:

开发的CRISPR/Cas12a集成纸质ELISA平台提供高灵敏度和快速检测.
该平台显示了临床诊断的潜力,克服了现有的纸质分析的局限性.
这种方法有助于在复杂的生物样本中敏感检测非核酸点.

关键词:

这就是CRISPR/CasPR.艾丽莎·伊丽莎·伊丽莎光是一种光.免疫测试是一种免疫测试.基于纸张的分析设备.

更多相关视频

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...