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优化和比较研究Bacillus联盟的细胞分解潜力和细胞酶酶活性
Ogechukwu Bose Chukwuma1, Mohd Rafatullah1, Riti Thapar Kapoor2
1Environmental Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang, 11800, Malaysia.
研究人员从垃圾填埋场的液中分离了八种Bacillus菌株,形成联盟,以提高纤维溶解效率. 这些细菌联盟显示出通过协同生物转化将纤维素分解为有价值的产品的巨大潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 细胞生物质是反抗性的,需要酶分解.
- 垃圾填埋场液是具有有价值的酶活性的微生物的潜在来源.
研究的目的:
- 从垃圾填埋场液中分离和识别细胞分解性细菌菌株.
- 为增强细胞酶活性构建和评估细菌联盟.
- 优化条件以获得最大的纤维溶解效率.
主要方法:
- 从垃圾填埋场泄漏物中分离细胞分解性细菌.
- 使用16S rDNA测序识别细菌菌株.
- 构建和测试细菌联盟的细胞酶活性.
- 优化温度和pH的酶活性使用dinitro酸盐方法.
主要成果:
- 八种细菌菌株被确定为Bacillus物种,包括B. subtilis,B. pumilus,B. proteolyticus,B. paramycoides,B. cereus,B. altitudinis,B. niacin和B. thuringiensis. 这些细菌菌株包括在内.
- 联盟A (B. proteolyticus,B. subtilis,B. pumilus,B. paramycoides) 在45°C和pH6时显示出最佳活性,产生0.90U/ml细胞质.
- 联合体B (B. cereus,B. altitudinis,B. niacin,B. thuringiensis) 在38°C和pH6时表现出0.78U/ml的细胞酶活性.
- 联盟内部的协同作用显著增强了生物转化过程.
结论:
- 已识别的Bacillus菌株及其联盟具有显著的纤维素分解潜力.
- 与单个菌株相比,细菌联盟显示出更高的纤维解效率,突出显示了协同关系在生物转化中的重要性.
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