提升了用L-乳酸提取的巴西的In Vitro生物可访问性和抗癌活性
Caglar Doguer1, Fatma Duygu Ceylan2, Esra Capanoglu2
1Department of Nutrition and Dietetics, Faculty of Agriculture, Tekirdag Namik Kemal University, Tekirdag 59030, Türkiye.
Preventive nutrition and food science
|March 10, 2025
概括
与乙醇 (EtOH) 相比,L-乳酸 (LA) 显著提高了从巴西中提取的化合物的提取和生物可访问性. 这种改进的提取能提高的提升.
科学领域:
- 食品科学 食品科学 食品科学
- 自然产品 化学 化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 是一种天然树脂混合物,富含化合物,具有潜在的健康益处.
- 乙醇 (EtOH) 是用于提取的常见溶剂,但其提高生物可访问性的效率仍在争论中.
- 建议L-乳酸 (LA) 作为从天然产品中提取生物活性化合物的优质溶剂.
研究的目的:
- 为了比较L-乳酸 (LA) 与乙醇 (EtOH) 的有效性,提高巴西红色和绿色中的化合物的生物可访问性.
- 评估使用LA和EtOH.获得的 прополис提取物的体外抗癌潜力.
主要方法:
- 提取物的胃肠道消化 (口腔,胃,肠道阶段).
- 使用Folin-Ciocalteu测定方法量化总含量 (TPC).
- 通过CUPRAC和DPPH测定评估抗氧化能力.
- 使用HPLC-DAD.AD 确定特定的化合物.
- 使用MTT测定对肺和结肠癌细胞的抗癌作用的评估.
主要成果:
- 与EtOH提取物相比,LA прополис提取物在胃和肠道消化后表现出明显更高的TPC和抗氧化活性.
- 红色草LA提取物 (RP-LA) 显示出强烈的剂量依赖性细胞毒性,对肺癌和结肠癌细胞.
- RP-LA在1,750 ppm下降了85% - 90%的癌细胞存活率,而EtOH提取物没有显著的细胞毒性作用.
结论:
- L-乳酸 (LA) 是比乙醇 (EtOH) 更有效的溶剂,可以从巴西中提取生物活性化合物,从而提高其生物可访问性.
- 用LA提取的 прополис具有显著改善的抗氧化剂和体外抗癌特性,特别是在肺癌和结肠癌方面.
- 这些发现强调了LA作为一种有希望的溶剂,可以最大限度地提高的治疗潜力.
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