非典型的RXLR因子参与了Phytophthora cactorum的病变发生
Zeyang Zheng1, Juanjuan Liu1, Wenzhong You2
1Liaoning Institute of Economic Forestry, Dalian, 116031 China.
aBIOTECH
|March 10, 2025
概括
非典型的RXLR效应器在Phytophthora cactorum病原发生过程中起着至关重要的作用. 这项研究确定和描述了这些非典型的效应因子,揭示了它们在宿主操纵和植物疾病中的重要性.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 卵菌生物生物学 卵菌生物生物学
背景情况:
- 卵胞细胞分泌RXLR效应器来操纵宿主免疫力.
- 植物虫 (Phytophthora cactorum) 造成了农业的重大损失.
- 在P.cactorum中非典型的RXLR效应器仍未得到充分研究.
研究的目的:
- 在P. cactorum中识别和描述典型和非典型的RXLR效应器.
- 将P. cactorum的RXLRs与其他虫虫病原体的RXLRs进行比较.
- 研究非典型RXLRs在P.cactorum病原发生中的功能作用.
主要方法:
- 对RXLR效应器的全基因组识别.
- 在oomycete物种中对RXLR曲目进行比较分析.
- 对RXLRs的基因表达分析.
- 在Nicotiana benthamiana中进行过渡表达测试,以评估效应器功能.
主要成果:
- 与其他Phytophthora物种相比,P. cactorum拥有较少的RXLR,但异型的比例较高.
- 大多数已识别的RXLR基因被转录,表明了潜在的功能.
- 非典型的RXLRs诱导了N. benthamiana的亡和抑制了宿主防御反应.
- 非典型的RXLRs促进了P.cactorum感染.
结论:
- 非典型的RXLRs对于P.cactorum的发病过程至关重要.
- 这项研究提供了关于P. cactorum非典型RXLRs的进化和宿主相互作用的见解.
- 这些发现有助于理解虫与植物的相互作用,并制定疾病管理策略.
更多相关视频
09:07Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
Published on: June 21, 2016
8.2K
07:41A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
8.8K
相关概念视频
Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
The Two-State Receptor Model
1.9K
The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with...
The binding affinity of a drug determines its interaction with...
1.9K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
GPCR Desensitization
5.7K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
5.7K
Spare Receptors
3.4K
Some receptors remain unoccupied even when an agonist produces a maximal response. Such empty ones are called spare receptors. In presence of spare receptors the maximum effect of an agonist drug is achieved with fewer than 100% of the receptors being occupied. To determine the presence of spare receptors, scientists often compare the concentration of the drug needed to produce 50% of the maximum effect (EC50) with the concentration of the drug needed to occupy 50% of the receptors (Kd). If the...
3.4K
Drug-Receptor Interaction: Agonist
2.3K
Agonists are drugs that interact with specific receptors in the body to produce a biological response. When an agonist binds to a receptor, it activates or enhances the receptor's function, leading to physiological effects. The interaction between agonist drugs and receptors is crucial for their therapeutic action in various medical treatments.
Agonists can bind to receptors in different ways. Some agonists bind directly to the receptor's active site, mimicking the endogenous...
Agonists can bind to receptors in different ways. Some agonists bind directly to the receptor's active site, mimicking the endogenous...
2.3K
