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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 7, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

波利泰克:用于多色数字PCR数据的自动聚类和标签方法.

Yao Chen^1,2,3, Ward De Spiegelaere^1,3,4, Wim Trypsteen^1,3,4,5,6

¹Digital PCR Center (DIGPCR), Ghent University, 9820 Merelbeke, Belgium.

NAR genomics and bioinformatics

|March 10, 2025

概括

此摘要是机器生成的。

波利泰克通过自动合并和标记集群来完善数字PCR (dPCR) 分析,改善核酸量化. 这种先进的方法提高了多色dPCR数据的准确性,超出了现有的技术.

更多相关视频

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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

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相关实验视频

Last Updated: Jun 7, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

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Published on: February 5, 2014

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

科学领域:

分子生物学分子生物学
生物技术是生物技术.
生物信息学是一种生物信息学.

背景情况:

数字聚合酶连锁反应 (dPCR) 是核酸量化的一种精确方法.
目前的自动化dPCR分析方法通常仅限于单色或双色数据.
现有的多色dPCR集群方法无法利用对集群位置的先验知识.

研究的目的:

推出Polytect,一种用于多色dPCR的自动分区分类的新方法.
提高dPCR数据分析的准确性和效率,特别是在复杂的多色分析中.
提供一个强大的工具,通过使用先前的知识来完善现有的集群结果.

主要方法:

波利泰克利用flowPeaks的原始集群结果,并通过自动集群合并和标签来改进它们.
该方法结合了对集群中心位置的先前知识,以改进分类.
通过使用实证和模拟数据,对现有流量峰,dpcp和ddPCRclust等方法进行了比较分析.

主要成果:

在经验和模拟的dPCR数据上,Polytect在与已建立的方法相比,表现优越.
该方法有效地合并多余的集群,并在适用时识别缺失的集群.
波利泰克成功地将dPCR分析扩展到多色数据,与仪器进步保持一致.

结论:

在自动化dPCR分区分类方面,Polytect提供了显著的进步,特别是在多色应用中.
该方法通过改进集群分析来提供更准确和可靠的核酸量化.
波利泰克可作为R包和R Shiny应用程序提供,以促进其在研究中的采用.