相关实验视频

Updated: May 23, 2025

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Published on: September 11, 2017

在植入前胚胎发育过程中,与几何学,细胞周期和母体转化为胚胎转化相关的YAP动态

Madeleine Chalifoux^1,2, Maria Avdeeva³, Eszter Posfai¹

¹Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.

bioRxiv : the preprint server for biology

|March 10, 2025

概括

早期哺乳动物胚胎发育涉及由Hippo路径和YAP驱动的细胞命运决定. 这项研究揭示了YAP水平在细胞分裂和定位过程中如何动态变化,从而影响细胞命运. 机械力量不会改变YAP的定位.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
遗传学遗传学是一个

背景情况:

哺乳动物胚胎中的第一个细胞命运决定是分离内部细胞质量 (ICM) 和皮 (TE) 血统.
河马信号通路及其效应器YAP调节了特定基因表达的基因表达.
在早期胚胎发生过程中,YAP对细胞变化的动态反应尚未完全理解.

研究的目的:

研究早期哺乳动物细胞命运分离过程中YAP局部化和水平如何动态变化.
了解YAP,细胞几何学和细胞周期进展之间的相互作用.
阐明确定YAP水平对ICM命运至关重要的机制.

主要方法:

在哺乳动物胚胎中的内源标记YAP的定量实时成像.
同时监测细胞几何特征和细胞周期进展.
应用概率模型来分析动态YAP和细胞几何数据.

主要成果:

YAP水平受到母亲提供的YAP,细胞周期和分裂后定位延迟的影响.
建立ICM命运的低核YAP在很大程度上是由被动细胞周期机制驱动的.
导致细胞形状变化的机械干扰不会影响胚胎中的YAP定位.

结论:

在早期胚胎细胞命运决定过程中,YAP水平受到多种因素的动态调节.
与细胞周期相关的机制在确定ICM命运的YAP水平方面发挥着重要作用.
这项研究提供了关于发育时间和细胞动态如何塑造YAP在谱系规范中的作用的见解.

关键词:

哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈细胞的命运细胞的命运动态贝叶斯网络是一个贝叶斯网络.现场成像现场成像现场成像这里是鼠标鼠标鼠标鼠标鼠标鼠标.在植入前的植入前.

更多相关视频

Imaging Cell Shape Change in Living Drosophila Embryos

Imaging Cell Shape Change in Living Drosophila Embryos

Published on: March 30, 2011

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Published on: December 20, 2021

相关概念视频

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...