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Updated: May 23, 2025

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实时和特定站点的动态亚细胞区间的扰乱使用Femtosecond脉冲

Seohee Ma^1,2, Bin Dong^1,2, Matthew G Clark¹

¹James Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, IN 47907, USA.

bioRxiv : the preprint server for biology

|March 10, 2025

概括

五秒激光器为生物研究提供精确的亚细胞控制. 一个名为fs-RPOC的新系统可实现细胞内部动态分子的自动实时准和扰动,进步了光学显微镜和光疗法.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.
光学工程是指光学工程.

背景情况:

连续波 (CW) 激光器使用线性吸收,而 Femtosecond (fs) 激光器使用非线性多光子吸收来实现精确的光学准.
目前的fs激光传递缺乏动态分子的自动定位和同时成像/扰动,阻碍实时生物分子研究.

研究的目的:

开发使用fs激光器进行自动化,化学选择性和实时亚细胞扰动的系统.
克服针对动态分子实体的局限性,并将激光传递与细胞成像相结合.

主要方法:

引入fs实时精密光学控制 (fs-RPOC),集成激光扫描显微镜与闭环反.
使用脉冲采集方法,在亚细胞位置独立控制激光平均和峰值功率.
准线粒体,研究对fs激光诱导的活性氧物种 (ROS) 和血生成的分子反应.

主要成果:

fs-RPOC在单个和子器官微手术中表现出卓越的空间精度和快速响应时间.
实现了局部分子调制,并揭示了线粒体中特定位置的分子反应.
在目标线粒体内观察到ROS形成,H2O2扩散和低密度血生成.

结论:

fs-RPOC能够在活细胞内进行精确的分子和器官调节.
提供了对fs激光与亚细胞区相互作用的新见解.
通过实时细胞控制突出了光学显微镜,光疗和光遗传学的发展潜力.

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