相关实验视频

Updated: May 23, 2025

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

高通量离心力显微镜揭示了单细胞水平上的动态免疫细胞贪

Hans T Bergal^1,2, Koji Kinoshita^1,2, Wesley P Wong^1,2,3,4

¹Program in Cellular and Molecular Medicine, Boston Children's Hospital.

bioRxiv : the preprint server for biology

|March 10, 2025

概括

一种新的离心力显微镜 (CFM) 量化了免疫细胞结合的强度和持续时间. 这种高通量方法揭示了双特异性T细胞参与者 (BiTE) 分子如何增强免疫细胞对癌症治疗的热情.

科学领域:

免疫学免疫学免疫学
生物物理学的生物物理.
细胞力学细胞力学

背景情况:

细胞与细胞的结合对于免疫反应至关重要,如监视和T细胞激活.
目前用于测量细胞度的方法缺乏吞吐量或精度.
双特异性T细胞激活剂 (BiTEs) 是一种新型免疫疗法,可以增强免疫细胞对癌症的向.

研究的目的:

开发一种高通量量化方法来测量细胞结合寿命和强度.
使用这种新方法,研究由BiTE分子介导的免疫细胞相互作用.

主要方法:

开发下一代离心力显微镜 (CFM) 具有多通道光成像.
利用CFM进行高通量,实时监测单细胞相互作用的强力.
进行细胞-蛋白结合和细胞-细胞激发性测试.

主要成果:

量化T细胞和B细胞从BiTE功能化的表面脱离,显示依赖于联体度的结合强度.
在细胞对细胞测试中显示出BiTE介导的免疫细胞狂热度的强烈,时间依赖的增加.
揭示了免疫细胞结合动态中的受体特异关系.

结论:

CFM提供了免疫相互作用的高通量,定量单细胞力分析.
这项技术为免疫细胞在强力下结合的动态提供了新的见解.
这些发现对免疫疗法开发和理解细胞力学有着广泛的影响.

更多相关视频

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Traction Force Microscopy to Study B Lymphocyte Activation

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...