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从转录凝聚物的逐步组装中出现的3D基因组重组

Surabhi Chowdhary¹, Sarah Paracha¹, Lucas Dyer¹

¹Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.

bioRxiv : the preprint server for biology

|March 10, 2025

概括

在酵母热冲击反应期间,转录凝聚物逐步组装以控制基因表达和3D基因组组织. 这一级联涉及转录因子,协活性剂和RNA聚合酶II,影响基因激活和基因组结构.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

转录凝聚物对于基因表达至关重要,但它们的组装和功能尚未完全理解.
了解凝聚物动力学是解读基因调节机制的关键.

研究的目的:

为了阐明在酵母中转录凝聚物的逐步组装.
为了研究这些凝结物在热冲击反应期间对基因控制和基因组组织的作用.

主要方法:

凝聚物形成的分子剖析.
对转录因子 (Hsf1) 突变的分析.
评估基因激活和3D基因组结构.

主要成果:

转录凝聚物聚集在一个级联中:hsf1聚类,调解器招募和Pol II凝聚.
基因激活是分级的,而不是开关式的,并且可以从凝结物形成中解开.
完全组装的冷凝剂促进了适应性反应的3D基因组重新配置.

结论:

转录凝聚物顺序组装,使得基因的分级控制.
凝结物形成的作用超出了直接的基因激活,影响了3D基因组组织.

关键词:

三维基因组的3D基因组生物分子凝结物是生物分子的凝结物.这就是Hsf1的意思.在Hsp70中,Hsp70是Hsp70.调解人调解人调解人在 RNA Pol II 中.陪伴者是一个陪伴者.基因转录基因转录热冲击反应的热冲击反应酸化的方法是:光化.蛋白质稳定症是一种蛋白质稳定症.

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