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在生物处理中选块共聚合物膜的多功能制造路线

Ke Meng^1,2, Alberto Alvarez-Fernandez³, Stefan Guldin^2,4,5

¹Department of Biochemical Engineering, University College London, Gower Street, London WC1E 6BT, U.K.

|March 10, 2025

概括

研究人员开发了一种新方法,用于制造异孔块共聚合物膜用于过. 这种技术为生物制药应用提供了更好的选择性,可能降低制造成本.

科学领域:

材料科学材料科学材料科学
化学工程是化学工程的重要组成部分.
生物技术是生物技术.

背景情况:

传统的聚乙硫 (PES) 膜在选择性-透性方面存在局限性,原因是孔径异质.
块共聚合物 (BCP) 膜具有可调节的特性,但它们通过非溶剂诱导相分离 (SNIPS) 进行制造是复杂且缓慢的.
需要有效的方法来制造异孔膜,用于先进的过应用.

研究的目的:

开发一种简化和快速的制造方法,用于异孔块共聚合物膜.
评估这些新型膜在过应用中的性能,特别是用于生物制药加工.
为了比较新膜的性能与商用聚硫 (PES) 膜.

主要方法:

在阳极氧化 (AAO) 支上采用了使用聚乙烯 (PS-b-PMMA) 块聚甲酸 (PS-b-PMMA) 的新型直接旋转涂层方法.
制造过程涉及将BCP薄膜造到一个充满水的AAO支上,以形成一个异孔结构.
通过测量诸如牛血清白蛋白,单克隆抗体和铁血球蛋白等生物分子的传播率来评估性能.

主要成果:

这种新方法成功地产生了异孔PS-b-PMMA薄膜复合膜.
这些膜显示牛血清白蛋白和单克隆抗体的传播率与商业PES膜相比相当.
观察到甲状腺球蛋白排斥显著改善了9倍,这表明切断精度提高了.

结论:

在AAO支上直接对BCP的自旋涂层提供了一条简化和高效的通往等孔膜的路线.
这些新型膜在拒绝更大的分子方面表现出卓越的表现,例如铁血球蛋白,这对于生物制药净化至关重要.
开发的膜具有提高单克隆抗体生产效率和降低成本的潜力,通过尽量减少染色学抛光步骤.

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