在棕藻的基因组内,多种多样的内源性巨型病毒元素存在
Dean Mckeown1,2,3, Alexandre Cormier4, Declan Schroeder3
1CNRS, Sorbonne Université, UPMC University Paris 06, Algal Genetics Group, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Place Georges Teissier, CS90074, Roscoff F-29688, France.
Virus evolution
|March 10, 2025
概括
在淡水棕色藻类Porterinema fluviatile中发现了来自不同病毒亚群的内源性病毒元素 (EVE). 一些EVE显示转录活动,表明正在进行的基因组整合.
科学领域:
- 基因组学就是基因组学.
- 病毒学 病毒学
- 海洋生物学 海洋生物学
背景情况:
- 内源性病毒元素 (EVE) 是在真核生物基因组中发现的集成病毒序列.
- 棕色藻类 (Phaeophyceae) 经常因感染核细胞病毒 (NCV) 病毒,特别是可以插入宿主DNA的菌病毒而携带EVE.
- 淡水棕色藻 Porterinema fluviatile 作为研究该群体EVE的模型.
研究的目的:
- 在淡水棕色藻 Porterinema fluviatile 的基因组中识别和描述内源性病毒元素 (EVE).
- 为了研究这种藻类内发酵病毒衍生的EVE的多样性和遗传关系.
- 评估这些EVE的转录活性和内源化潜力.
主要方法:
- 对Porterinema fluviatile基因组进行生物信息分析,以检测插入的病毒序列.
- 遗传学分析以确定已识别的EVE的起源和关系.
- 转录组分析以评估EVE中的基因表达.
主要成果:
- 确定了七个大型EVE,包括四个完整或近乎完整的前病毒,起源于独立的插入事件.
- 这些EVE代表了来自A和B分组的基因组学上多样化的Phaeovirus成员,这是一个令人惊的发现,因为它们的进化策略被认为是不同的.
- 大多数EVE基因在转录上是无声的,但有些基因表现出活性,还有一些基因含有内子,表明潜在的正在进行的内源化.
结论:
- 淡水棕色藻 Porterinema fluviatile 藏了一系列来自病毒的EVE.
- 来自不同病毒亚群的EVE的存在挑战了关于它们进化差异的先前假设.
- 转录活动和内子的证据表明,这些EVE可能正在融入宿主基因组的过程中.
关键词:
病毒 (phaeovirus) 是一种病毒.波特里尼马流的流动性棕色藻类是一种色藻类.内生化 (endogenization) 是一种内生化过程.它们是内源性病毒元素.基因组 基因组是基因组的组成部分.更多相关视频
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