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在口腔状细胞癌中,巨细胞 - 瘤细胞相互作用相关的基因和微RNA表达特征

Tatjana Khromov^1,2, Maren Sitte³, Gabriela Salinas³

¹Department of Clinical Chemistry, University Medical Center Goettingen, Goettingen, Germany.

Frontiers in oncology

|March 10, 2025

概括

巨细胞 (MCs) 和口腔状细胞癌 (OSCC) 细胞通过特定的信号通路和microRNAs (miRNAs) 相互作用. 针对这些相互作用,比如CCL2/CCR2轴,可能为头瘤提供新的治疗方法.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

乳腺细胞 (MCs) 是口腔状细胞癌 (OSCC) 的瘤微环境 (TME) 中的关键参与者,影响患者的治疗结果.
了解MC和OSCC细胞之间的分子对话对于开发有效的治疗方法至关重要.

研究的目的:

在共同培养期间研究MC和OSCC细胞的基因和microRNA (miRNA) 表达特征.
为了确定TME中MC-OSCC相互作用的基础分子机制.

主要方法:

人类OSCC (PCI-13) 和MC (LUVA) 细胞系的共同培养.
对差异表达基因 (DEGs) 和miRNA测序进行转录组分析.
生物信息学分析和评估头和部瘤的预后相关性.

主要成果:

在诸如化学激素信号传递 (CCL2/CCR2轴),TGF-β,TLR,PI3K/Akt,JAK/STAT,Ras/Raf/MAPK和IP3.3等途径中确定了差异性基因表达.
在瘤细胞中发现了包括miR-142,miR-146a,miR-223在内的miRNAs的显著差异表达,并在MC中发现了miR-381,miR-379.
确定CCR2,miR-142,miR-146a和miR-223在头部和部瘤中具有预后意义.

结论:

MCs和OSCC之间的相互作用是复杂的,由特定的信号通路和miRNAs调节.
这些发现为未来的功能研究和向治疗提供了基础,以调节TME中的MC-OSCC相互作用.

关键词:

CC 化学因子连接体 2 化学因子连接体 2 在CCL2中,我们可以使用CCL2.CCR2 是一个 CCR2 系统.这是OSCCCC的OSCC.巨细胞巨细胞口腔状细胞癌的癌症.预后预后预后瘤微环境是一个微环境.

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