介质淋巴变化的时空发育在终端炎的TNF小鼠模型中
Keith Keane1, Matthew Stephens1, Simon Roizes1
1Department of Physiology and Pharmacology, Inflammation Research Network, The Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
概括
在克罗恩病中,中腔淋巴变化逐步发展,从淋巴腺病和炎症开始. 肠道微生物组加剧了这些变化,推动了免疫细胞的聚集和淋巴细胞器官的形成.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
背景情况:
- 克罗恩氏病 (CD) 涉及肠道的慢性炎症和中腔淋巴系统的改变.
- 淋巴变化在CD病变发生过程中的确切作用尚不清楚.
研究的目的:
- 在CD型阴道炎的小鼠模型中,描述介质淋巴变化的空间和时间发展.
- 研究肠道微生物群对这些淋巴变化和相关炎症的影响.
主要方法:
- 使用TNFΔARE/+小鼠模型,比较不同年龄 (8,12,20,28周) 的特定无病原体 (SPF) 和无细菌 (GF) 条件.
- 通过髓氧化酶 (MPO) 活性评估乳头炎.
- 分析了使用共聚焦免疫光成像的中腔淋巴变化.
主要成果:
- 在SPF TNFΔARE/+小鼠中,淋巴细胞的变化逐步与阴道炎进展,首先是中腔淋巴腺病变,其次是淋巴管切开,淋巴管生成和三级淋巴体器官形成.
- 没有细菌的TNFΔARE/+小鼠表现出淋巴变化 (淋巴腺病变,淋巴管切开,淋巴管生成),没有结膜炎,免疫细胞聚类或三级淋巴体器官.
- 肠道微生物组的存在加剧了淋巴细胞的改变,对免疫细胞聚类和三级淋巴体器官的发展至关重要.
结论:
- 克罗恩病中介质淋巴变化逐渐发展,并受到肠道微生物群的影响.
- 虽然免疫细胞的透和淋巴细胞细胞生成取决于微生物组,但淋巴血管的变化是独立的.
- 这些发现突出了淋巴系统,炎症和克罗恩病中的微生物群之间的复杂相互作用.
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