Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advancing quantitative clinical pharmacology competencies in Francophone Africa through an on-line learning framework.

Journal of pharmacokinetics and pharmacodynamics·2026
Same author

Recommendations of the Polish Zebrafish Society on the use of the zebrafish (<i>Danio rerio</i>) model in biomedical research.

Acta biochimica Polonica·2026
Same author

Differential salivary proteome of nonsyndromic cleft lip and/or palate patients with and without dental caries in comparison with nonaffected individuals.

European journal of orthodontics·2026
Same author

High Nasopharyngeal SARS-CoV-2 Load and Delayed Clearance in Hospitalized Patients With Blood Autoantibodies Neutralizing Type I Interferons.

The Journal of infectious diseases·2026
Same author

Standardised Surface Electromyography During Clenching and Swallowing: Patient Perception and Comparison of Two Protocols.

Journal of oral rehabilitation·2026
Same author

Three-Dimensional Evaluation of Secondary Alveolar Bone Grafting in Patients With Bilateral Cleft Lip and/or Palate: A 2-3-Year Post-Operative Follow-Up.

Orthodontics & craniofacial research·2025

相关实验视频

Updated: Jul 3, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.0K

在Smad交互蛋白1 (Sip1) Null小鼠中出现缺陷切口器的发展.

Marie De Laet1, Julie Bertrand1, Elisa Vingerhoedt1

  • 1Department of Oral Health Sciences-Orthodontics, KU Leuven and Dentistry, University Hospitals Leuven, Leuven, Belgium.

Orthodontics & craniofacial research
|March 10, 2025
PubMed
概括

条件性Smad交互蛋白1 (Sip1) 基因突变在小鼠中会导致显著的牙和头骨面部形. 这项研究揭示了人类发育异常的潜在机制.

关键词:
在Prx1-Cre Sip1小鼠中,头面部和牙的异常情况.神经顶细胞是神经顶细胞.

更多相关视频

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
07:40

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

30.0K
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
10:03

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Published on: May 10, 2019

12.4K

相关实验视频

Last Updated: Jul 3, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

11.0K
Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
07:40

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

30.0K
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
10:03

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Published on: May 10, 2019

12.4K

科学领域:

  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个
  • 面生物学 面生物学

背景情况:

  • Smad交互蛋白1 (Sip1) 在胚胎发育中起着至关重要的作用.
  • 针对Sip1的向性失活导致胚胎死亡,需要条件突变模型.

研究的目的:

  • 在具有条件Sip1基因突变的新型小鼠模型中,在组织学和形态学上表征牙和面异常.
  • 研究Sip1在面和牙发育中的作用.

主要方法:

  • 利用Prx1-Cre小鼠模型进行组织特异性Sip1基因的非激活.
  • 分析了胚胎 (14.5-18.5 dpc) 和Sip1无细胞小鼠 (新生儿和5个月大) 使用免疫组织化学 (β-catenin,Ki67) 和形态检查.
  • 使用曼-惠特尼U测试,比较了淘汰和野生类型小鼠之间的dentofacial测量.

主要成果:

  • 切肢位置和形状的变化被观察到为15.5dpc.
  • 突变新生儿表现出扩大了形,下形下,状气膜过程,以及更短的切口.
  • 成年突变者表现出合的 sutures,低塑性部,以及延长的,曲的切口.

结论:

  • Sip1对于正常的牙和面部发育至关重要.
  • 有条件的Sip1突变导致了一系列的牙和头骨形.
  • 该模型提供了对人类面和牙异常的洞察力,可能有助于诊断和治疗.