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在ilvN基因中的突变减轻了Corynebacterium glutamicum中氨酸的增长抑制作用

Kazuho Matsuhisa¹, Katsuhiro Ogawa¹, Kento Komata¹

¹School of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta-cho, Midori-Ku, Yokohama, Kanagawa, 226-8501, Japan.

Applied microbiology and biotechnology

|March 10, 2025

概括

氨酸通过破坏氨酸生物合成来抑制微生物的生长. 在Corynebacterium glutamicum中分离出抗氨酸的突变,揭示了ilvN中的突变,增强了氨酸的产生,并改善了细胞生长,从而增加了氨酸的产生.

科学领域:

微生物学微生物学
生物化学生物化学
代谢工程是代谢工程.

背景情况:

氨酸是一种对食品,化品和制药行业至关重要的氨基酸.
氨酸对微生物细胞具有增长抑制作用,阻碍其产生.
微生物系统中囊诱导的生长抑制背后的机制尚不清楚.

研究的目的:

为了阐明Corynebacterium glutamicum中氨酸诱导的生长抑制的机制.
为了确定在C. glutamicum中赋予囊氨酸耐药性的遗传修饰.
评估囊抵抗作为增强囊生产的表型的实用性.

主要方法:

通过适应性实验室进化 (ALE) 来分离C. glutamicum的抗氨酸突变.
进化菌株的基因组重新排序以确定遗传突变.
对分支链氨基酸生物合成和乙酸酸合成酶 (AHAS) 活性进行分析.

主要成果:

编码AHAS的一个子单元的ilvN基因的突变在抗氨酸突变物中被发现.
ilvN突变导致氨酸的产生增加,并且在氨酸的存在下改善了细胞生长.
氨酸抑制了AHAS活性,而突变的AHAS则表现出耐药性,证实了氨酸生物合成扰乱的作用.

结论:

在C. glutamicum中,氨酸诱导的生长抑制主要是由于氨酸生物合成的干扰.
赋予氨酸抵抗性的突变,特别是在ilvN中,增强细胞生长和氨酸的产生.
囊抵抗是一种有价值的表型,用于在C. glutamicum中开发改善的囊生产宿主.

关键词:

在Corynebacterium glutamicum的研究中,我们发现这样一来,他们就会有更多的乐趣.适应性实验室进化的演变.半氨酸 (cysteine) 是一种蛋白质.增长抑制是一种增长抑制.氨酸 (Valine) 是一种氨酸.

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