酵母的真空细胞是一个特权的利基,可以保护细胞内细菌免受抗生素的侵害
Atousa Hatefi1, Farideh Siavoshi2, Saman Khalili-Samani1
1Department of Microbiology, School of Biology, University College of Sciences, University of Tehran, Tehran, Iran.
Archives of microbiology
|March 10, 2025
概括
抗生素没有消除Candida tropicalis酵母中的细胞内细菌. 酵母挤出了抗生素,即使在处理后,在它们的真空孔内保持了活跃的细菌.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在真核细胞内的细胞内细菌对抗微生物治疗提出了独特的挑战.
- 已观察到Candida tropicalis有各种细菌,包括Helicobacter pylori,Staphylococcus,诺卡迪亚和菌.
- 抗生素治疗在酵母真空中对这些细胞内细菌的疗效尚不清楚.
研究的目的:
- 为了研究抗生素混合物 (ABM) 在消除位于Candida tropicalis中的细胞内细菌方面的有效性.
- 为了确定酵母细胞是否可以排出抗生素,从而保护细胞内细菌.
主要方法:
- 含有细胞内细菌的Candida tropicalis酵母培养物用不同度的抗生素混合物 (阿莫西西林,西普罗夫洛克萨,里法,美特罗尼达) 进行治疗.
- 通过使用活/死染色和殖民地计数来评估细菌活力.
- 细菌16S rRNA基因的存在和完整性通过PCR进行了分析.
- 用显微镜检查了酵母细胞活力和形态.
主要成果:
- 抗生素治疗没有显著降低Candida tropicalis或其细胞内细菌的活力.
- 细菌16S rRNA基因在经过处理的酵母培养中始终被检测到,证实了细胞内细菌的持久性.
- 观察到酵母细胞挤出抗生素,减少它们的有效度并保护细胞内细菌.
- 完好无损的和受损的酵母细胞都在真空中藏着可活的细胞内细菌.
结论:
- 在Candida tropicalis的真菌真空中建立的细菌对抗生素破坏具有抵抗力.
- 热带菌 (Candida tropicalis) 活跃地挤出抗生素,损害了它们对细胞内细菌群的有效性.
- 目前的抗生素治疗方案在消除这种酵母菌种所携带的细胞内细菌方面是无效的.
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