葡萄糖补充剂对在热中性或热应激环境下养的的蛋白质生物合成的影响
Josephine Kwakye1, Oluwatomide W Ariyo1, Ahmed F A Ghareeb1
1NutriGenomics Laboratory, Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.
Gene
|March 10, 2025
概括
热应激对肉有负面影响,但补充葡萄糖可以增强蛋白质生物合成. 葡萄糖影响mTOR通路中的关键信号基因,在热应激条件下改善肉健康.
科学领域:
- 动物科学动物科学
- 营养生理学 营养生理学
- 分子生物学分子生物学
背景情况:
- brojlers中的热应激 (HS) 减少了料摄入量,损害了营养的可用性和蛋白质生物合成.
- 外源葡萄糖可能提供能量资源,以抵消HS对蛋白质合成的影响.
研究的目的:
- 为了研究外源葡萄糖对热应激下 brojlers 蛋白质生物合成的影响.
- 分析热应激和葡萄糖对哺乳动物目标拉巴素 (mTOR) 信号通路的影响.
主要方法:
- 四个治疗组:热中性 (TN) 和热应力 (HS),使用0%或6%的葡萄糖.
- 在为期7天的实验期内,使用了456只四周大的Cobb500肉.
- 在mTOR途径中对基因表达和蛋白质酸化的分析.
主要成果:
- 热应力改变了mTOR,S6K1和Foxo3a的酸化,表明了适应性反应.
- 葡萄糖补充剂 (HS6) 与没有葡萄糖 (HS0) 相比,增加了mTOR途径基因的mRNA表达,除了avTOR,Akt1和S6K1.
- 观察到鸟类目标拉巴胺素 (avTOR) 和与自相关的基因之间存在反向关系.
结论:
- 外源葡萄糖可以影响热应激下mTOR通路中关键信号基因的酸化状态.
- 葡萄糖补充剂可以通过调节热应激的肉mTOR途径来增强蛋白质生物合成.
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