Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

12.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
12.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Anti-CRISPR-Based and CRISPR-Based Genome Editing of <i>Sulfolobus islandicus</i> Rod-Shaped Virus 2.

Viruses·2018
Same author

Dissolution and homogeneous photocatalysis of polymeric carbon nitride.

Chemical science·2018
Same author

Mycophenolic Acid as a Promising Fungal Dimorphism Inhibitor to Control Sugar Cane Disease Caused by Sporisorium scitamineum.

Journal of agricultural and food chemistry·2018
Same author

A smart tumor microenvironment responsive nanoplatform based on upconversion nanoparticles for efficient multimodal imaging guided therapy.

Biomaterials science·2018
Same author

Understanding Membrane Protein Drug Targets in Computational Perspective.

Current drug targets·2018
Same author

The important role of apolipoprotein A-II in ezetimibe driven reduction of high cholesterol diet-induced atherosclerosis.

Atherosclerosis·2018

相关实验视频

Updated: May 10, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

艾丽莎蛋白质检测器 (EPD):基于Python的ELISA工具,用于准确的低水平蛋白质量测量.

You Lu1, Li Qi2, QinZheng Xu3

  • 1Department of Medicine, Tulane School of Medicine, Tulane University, New Orleans, LA 70112, USA.

Journal of immunological methods
|March 10, 2025
PubMed
概括

一个新的软件,ELISA蛋白质检测器 (EPD),改善了低度蛋白质在酶相关免疫吸收试验 (ELISA) 中的检测. 这种开源工具提高了研究蛋白质分泌的研究人员的数据分析准确性.

关键词:
在曲线上适应曲线.艾丽莎·伊丽莎·伊丽莎非线条最小平方的非线条最小平方蛋白质度量化的量化蛋白质度.这是一个基于Python的程序.

更多相关视频

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
09:56

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

相关实验视频

Last Updated: May 10, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
09:56

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Published on: October 13, 2021

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物分析化学 生物分析化学

背景情况:

  • 与酶相关的免疫吸收试验 (ELISA) 对于量化蛋白质分泌至关重要.
  • 由于校准曲线的适配限制,标准ELISA软件往往无法准确检测低蛋白度 (亚纳米/毫升).

研究的目的:

  • 开发一种开源软件解决方案,即ELISA蛋白检测器 (EPD),以克服标准ELISA软件在检测低度蛋白质方面的局限性.
  • 提高ELISA数据分析的精度和可靠性,特别是在低检测值时.

主要方法:

  • 开发了EPD作为一个开源的基于Python的软件.
  • 实现了先进的优化算法,以提高曲线拟合精度.
  • 设计了一个直观的用户界面,最小的技术专业知识要求.
  • 整合了强大的交叉验证功能,以提高数据可靠性.

主要成果:

  • EPD在拟合校准曲线方面表现出更高的精度,特别是在亚纳克/毫升蛋白质度方面.
  • 该软件提供了低水平蛋白质度的准确量化,超过了标准的ELISA阅读器软件.
  • 在Windows系统上进行了测试,EPD为研究人员提供了具有成本效益和多功能性的解决方案.

结论:

  • 在低度蛋白质检测方面,EPD有效地解决了传统ELISA软件的缺陷.
  • 该工具可以在各种生物和临床研究中更可靠,更准确地量化蛋白质分泌.
  • EPD赋予研究人员先进的分析能力,用于敏感蛋白质分析.