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通过滚动圆放大 (RCA) 克隆完整的双子病毒基因组

Francisco Murilo Zerbini1

  • 1Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil. zerbini@ufv.br.

Methods in molecular biology (Clifton, N.J.)
|March 11, 2025
PubMed
概括

滚动圆放大 (RCA) 是一种简单的同热方法,可以在没有先前序列知识的情况下放大圆形DNA. 这种技术对于克隆完整的双胞胎病毒基因组至关重要,使得双胞胎病毒学领域的广泛研究成为可能.

关键词:
开始病毒病毒.美国大肠杆菌 (E. coli).一个全长的克隆.这是一种等离子体.在RCA RCA中使用.

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