相关实验视频

Updated: May 23, 2025

Mouse Wound Models and Preparation of Single-Cell Suspensions

Mouse Wound Models and Preparation of Single-Cell Suspensions

Published on: September 27, 2024

人类诱导的多能干细胞衍生的外体细胞通过激活FGF2-介导的p38通路促进皮肤伤口愈合

Rongrong Zhang^1,2, Huilan Wu¹, Yongmiao Peng¹

¹Basic Medical Research Center, The Second Affiliated Hospital and Yuying Children'S Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, China.

Molecular and cellular biochemistry

|March 11, 2025

概括

人类诱导的多能干细胞衍生的外体细胞 (hiPSC-Exos) 通过减少炎症和促进细胞生长和迁移来加速急性皮肤伤口愈合. 这些外体可能通过向FGF-2/FGFR3/p38通路来提供有希望的无细胞治疗皮肤修复.

科学领域:

再生医学是一种再生医学.
生物技术是生物技术.
皮肤病学皮肤病学

背景情况:

急性和大面积的皮肤愈合仍然是一个重大的临床挑战.
无细胞疗法,如从人类诱导的多能干细胞 (hiPSC-Exos) 衍生出的外体,显示出对皮肤损伤修复的希望.

研究的目的:

研究hiPSC-Exos对皮肤急性伤口愈合的作用,体内和体外.
探索hiPSC-Exos在皮肤修复中的潜在调节机制.

主要方法:

建立了体内 (小鼠的全层皮肤损伤) 和体内 (角质细胞划伤模型) 创伤愈合模型.
使用PKH26标签来跟踪hiPSC-Exos吸收.
评估了炎症标志物 (mRNA表达),细胞增殖 (PCNA,EDU) 和细胞迁移.
使用特定抑制剂 (AZD4547,SB203580) 调查了纤维细胞生长因子受体3 (FGFR3) 和p38通路的作用.

主要成果:

hiPSC-Exos被伤口区域的细胞有效地吸收.
hiPSC-Exos通过抑制炎症因子 (Il-1β,Ccl2,Cxcl5,Ccl7) 和增加细胞增殖 (PCNA,EDU) 来加速伤口愈合.
hiPSC-Exos促进了角质细胞迁移,这种效应被FGFR3和p38抑制剂逆转.
hiPSC-Exos含有FGF-2并通过FGFR3激活p38通路,而不会影响ERK或JNK通路.

结论:

hiPSC-Exos通过减少炎症和增强细胞增殖和迁移,促进急性皮肤伤口愈合.
该机制涉及hiPSC-Exos中固有的FGF-2,准FGFR3以激活p38通路.
hiPSC-Exos代表了一个有前途的无细胞治疗候选人,用于皮肤愈合.

关键词:

衍生出异体体 (hiPSC,Exos) 是一种纤维细胞生长因子2 (FGF,2)人类诱导的多能干细胞干细胞.移民移民移民核武器的扩散皮肤伤口愈合的愈合

更多相关视频

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Published on: August 21, 2020

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: April 18, 2019

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...