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Updated: May 23, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

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使用转录延长复合显示器对共转录RNA折叠的系统分析

Skyler L Kelly1, Eric J Strobel2

  • 1Department of Biological Sciences, The University at Buffalo, Buffalo, NY, 14260, USA.

Nature communications
|March 11, 2025
PubMed
概括

一种新的高通量方法,转录延长复合显示 (TECdisplay),通过测量转录期间新生的RNA活动来评估RNA功能. 这个平台有效地分析数百万个RNA变体,揭示了RNA结构-功能关系的洞察力.

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Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
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Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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