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凝MA作为上皮细胞的支架材料,以模拟小肠微环境

Inez Roegiers^1,2, Tom Gheysens³, Manon Minsart³

¹Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. inez.roegiers@ugent.be.

Scientific reports

|March 11, 2025

概括

凝甲基胺 (GelMA) 水凝显示出作为肠道上皮细胞的支架的前景. 它们为体外肠道模型提供良好的透性,机械强度和生物相容性,支持宿主微生物相互作用研究.

科学领域:

生物材料科学生物材料科学
胃肠病学胃肠病学
组织工程是组织工程.

背景情况:

肠道中的宿主微生物相互作用对健康和疾病至关重要.
目前的体外模型需要改进的支架,以准确模仿肠道环境.
凝甲烯胺 (GelMA) 被探索为一个潜在的支架材料.

研究的目的:

评估GelMA作为肠道上皮细胞的支架.
根据透性,机械强度和生物相容性来评估GelMA的适用性.
为了比较GelMA水凝的灭菌方法.

主要方法:

通过使用生物相关标记物,研究了GelMA对透性的替代程度 (DS) 影响.
评估了不同度的GelMA的细胞粘附性,活力和水凝机械性能.
乙烯氧化物 (EO) 和乙醇/UVC灭菌方法对 GelMA 的特性和细胞反应进行了比较.

主要成果:

高和低的DS GelMA都显示出足够的传输标记的透性.
凝MA水凝支持Caco-2和LS174T细胞的长期细胞活力和粘附性.
凝MA的机械性能在肠道支架的生理范围之内.
绝育方法 (EO与EtOH/UVC) 对GelMA的机械性质和细胞活力影响很小.

结论:

凝MA是适合肠道细胞类型的支架材料,满足体外建模的关键要求.
这些发现推动了更多可翻译的肠道体外模型的开发,用于研究宿主微生物相互作用.

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