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Updated: May 23, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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在金纳米颗粒上固定的DNA的密度和结构会影响核酸检测中的灵敏度

Nanami Fukuzumi1, Gen Hirao1, Atsushi Ogawa2

  • 1Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan.

Scientific reports
|March 11, 2025
PubMed
概括

在金纳米颗粒 (AuNPs) 上优化DNA密度和结构可以提高它们作为遗传诊断生物传感器的使用. 降低DNA密度可以提高线性DNA的检测灵敏度,但对刚性结构的影响不同.

关键词:
聚合是一种聚合.固定DNA的密度和结构.对盐的分散稳定性.黄金纳米颗粒的使用方法核酸检测检测核酸检测的方法

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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