含有nifH的有机体在海洋寒冷海域的丰富性,身份和潜在的亚松活动
Amanda C Semler1, Emily R Paris1, Mikaela Salvador2
1Department of Earth System Science, Stanford University, Stanford, California, USA.
Environmental microbiology
|March 11, 2025
概括
海洋冷的透点是缩的热点,各种微生物固定. 在漏中,高酶基因 (nifH) 的丰富性表明了显著的生物固化活性.
科学领域:
- 海洋微生物学 海洋微生物学
- 生物地质化学生物地质化学
- 分子生态学分子生态学
背景情况:
- 隔离体微生物对于气循环在气有限的环境中至关重要,如海洋冷.
- 由nifH基因编码的酶是生物固化的关键酶.
研究的目的:
- 调查海洋冷中的含有nifH的有机体 (NCOs) 的多样性和丰度.
- 评估这些入物体内和外的二氧化活动的流行程度.
- 为了评估环境因素对缩的影响.
主要方法:
- 对 nifH 基因的Amplicon 测序.
- 定量实时PCR (qPCR) 对于nifH基因和转录丰度.
- 在六个生态化学异质的冷中分析NCOs.
主要成果:
- 检测到18个细菌和考古学科的各种NCO,其中17个在seeps.com中发现.
- 已识别的NCO包括甲氧化古生物和硫酸盐减少细菌.
- 观察到广泛的nifH转录,表明在六个漏中的五个处有活跃的二氧化缩.
- 经过校正的qPCR数据显示,入物体内的nifH丰度高出三倍.
- 发现nifH的丰度与孔水氨有负相关性,表明抑制.
结论:
- 海洋寒冷的透藏着一个多样化社区的可食性微生物.
- 沉是深海生物固化的重要热点.
- 环境因素,特别是的度,影响了亚类活动.
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