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相关概念视频

Structure of a Gene01:30

Structure of a Gene

12.3K
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
12.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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2.9K
Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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相关实验视频

Updated: May 23, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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基因表达动态的Tragedy轨迹对齐

Ross F Laidlaw1, Emma M Briggs1,2,3, Keith R Matthews2

  • 1Centre for Parasitology, University of Glasgow, Glasgow, G12 8QQ, United Kingdom.

Bioinformatics (Oxford, England)
|March 11, 2025
PubMed
概括

基因表达动态的轨迹对齐 (TrAGEDy) 从单细胞测序数据准确地对齐不对称的生物过程. 这种新方法的性能优于现有的工具,在复杂的发育研究中揭示了更多生物学相关的基因和途径.

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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
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科学领域:

  • 计算生物学 计算生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 单细胞转录组学可以在生物过程中进行比较.
  • 在单细胞数据中整合不对称的过程是当前方法的挑战.
  • 现有的方法往往需要在分析之前进行样本整合,冒着错误的风险.

研究的目的:

  • 引入基因表达动态 (TrAGEDy) 的轨迹对齐,用于对齐独立的单细胞轨迹.
  • 克服当前处理不对称生物过程的方法的局限性.
  • 用单细胞数据改进复杂生物系统的分析.

主要方法:

  • TrAGEDy对准了独立的轨迹,绕过了样本集成的需要.
  • 该方法已在R中实现,并可免费使用.
  • 在模拟和真实生物数据集上评估性能.

主要成果:

  • TrAGEDy准确地恢复模拟数据中的基本对齐,超过现有工具的性能.
  • 该方法成功地捕获了其他方法错过的复杂不对称对齐.
  • 对T细胞发育和Trypanosoma brucei淘汰数据的应用确定了更多相关的基因和过程.

结论:

  • 特拉格迪提供了一个强大的解决方案,用于整合不对称的单细胞轨迹.
  • 该工具增强了在复杂的发育研究中发现生物学意义上的发现的发现.
  • 在比较生物学中,Tragedy为单细胞数据分析提供了宝贵的进步.