细菌菌 Fragilis 中的原体:分布和遗传多样性
Paolo Gaibani1,2, Rocco Latorre3,4
1Department of Diagnostic and Public Health, Microbiology Section, University of Verona, Verona, Italy.
Heliyon
|March 11, 2025
概括
前体在 Bacteroides fragilis 基因组中普遍存在,并观察到显著的保护性. 这种多样性可能表明病原菌株的适应性,以及人类肠道内的共生菌株的稳定性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 细菌菌 (Bacteroides fragilis) 是人类关键的肠道共生物.
- 前体,病毒DNA集成到细菌基因组中,在细菌进化和适应中发挥作用.
- 了解prophage分布和特征对于破译细菌种群动态至关重要.
研究的目的:
- 为了研究细菌菌群 Bacteroides fragilis 基因组内的传染区域的分布,流行病学和基因组关系.
- 描述不同B. fragilis菌株的prophage序列及其保存.
- 为了与细菌菌株分裂和毒素基因的存在相关联的prophage特征.
主要方法:
- 对500个 Bacteroides fragilis 群体基因组进行全基因组分析.
- 平均核酸标识 (ANI) 用于物种级别的表征.
- 遗传学分析以确定基因组关系.
- 使用PHASTEST进行原体鉴定,并与Jspecies进行对对比.
主要成果:
- 在67.6%的B. fragilis基因组中发现了原体,其中54.2%的区域具有高核酸同一性 (>80%).
- 第I类菌株比第II类菌株含有更多的prophages (p < 0.01).
- 与II类菌株 (71.88% vs 90.0%;p < 0.0001) 相比,I类菌株的prophage序列保存率较低 (≥90%的同一性).
- 携带毒素的菌株表现出比毒素阴性菌株 (70.0%对95.4%;p < 0.0001) 的较低的prophage保存率 (≥70%的同一性).
结论:
- 前体被广泛分布,并且在 Bacteroides fragilis 基因组中高度保存.
- 观察到的prophage多样性表明了致病菌株的适应性.
- 在开始性菌株中,菌区的高度保存可能与人类肠道环境中的低选择性压力有关.
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