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马中性粒细胞选择性地释放中性粒细胞外陷,以应对化学和细菌激动剂

Breanna J Sheahan^1,2, Alicia G Schubert¹, William Schubert³

¹Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.

Frontiers in veterinary science

|March 11, 2025

概括

马中性粒细胞在对细菌和化学刺激的反应中形成中性粒细胞外陷 (NETs). 无论是NOX依赖的路径还是NOX独立的路径,与自一起,都调节马匹NET的形成,为马匹疾病提供治疗点.

科学领域:

免疫学免疫学免疫学
兽医医学兽医医学兽医医学
细胞生物学细胞生物学

背景情况:

中性细胞外细胞陷 (NETs) 对于对抗感染至关重要,但可以加剧炎症性疾病.
了解特定物种的NET调节对于治疗干预至关重要,特别是在对细菌刺激敏感的马群中.

研究的目的:

为了比较马类中性粒细胞对各种刺激的反应 (PMA,A23187,S. aureus,E. coli).
研究NADPH-氧化酶 (NOX) 和自在马类NET形成中的作用.

主要方法:

用PMA,A23187,S. aureus和E. coli刺激马类中性粒细胞.
评估了NOX路径的参与.
用自抑制来研究其对NET释放的影响.

主要成果:

马中性粒细胞在对A23187和大肠杆菌的反应中产生了显著的NET,对PMA和S. aureus的反应较低.
无论是依赖NOX的路径还是不依赖NOX的路径,都会导致马匹的NET形成.
自抑制减少了在NET释放过程中细胞外DNA串流的发生.

结论:

马中性粒细胞对不同的NET诱导刺激表现出不同的反应.
氧和自是马匹NET形成的关键调节者.
研究结果为管理喘和乳膜炎等马类炎症状况提供了洞察力.

关键词:

通过NADPH氧化酶路径.自自是自的过程.素基因组合素的素基因组合素.马类的马类动物.中性粒细胞外细胞陷中性粒细胞中性粒细胞.

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