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在Dendrobium huoshanense的各种压力治疗下对CYP450s进行全基因组识别和表达分析

Guohui Li¹, Xingen Zhang¹, Yuyue Li¹

¹Traditional Chinese Medicine Institute of Anhui Dabie Mountain, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012 China.

Physiology and molecular biology of plants : an international journal of functional plant biology

|March 11, 2025

概括

这项研究在Dendrobium huoshanense中确定了193个P450 (CYP450) 基因,揭示了它们的分布和调节元素. 这些基因对寒冷,干旱和激素做出反应,对植物生长和应激适应至关重要.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

细胞染色体P450单氧化酶 (CYP450) 是主要和次要新陈代谢的重要植物酶.
缺乏对Dendrobium物种中CYP450s的系统性表征.

研究的目的:

为了识别和描述Dendrobium huoshanense中的CYP450基因家族.
研究 DhCYP450 基因在植物生长和应激反应中的潜在作用.

主要方法:

用于基因识别和分类的生物信息分析.
染色体定位和图案分析.
促使者cis作用元素的预测.
定量实时PCR (qRT-PCR) 用于表达式分析.

主要成果:

193个DhCYP450基因被确定并分为2个组和10个氏族.
基因分布在19个染色体中,发现了8个保存的基因.
促使者分析揭示了对低温,干旱和荷尔蒙反应的元素.
qRT-PCR证实了对非生物压力和激素的反应中的基因表达.

结论:

DhCYP450基因家族在Dendrobium huoshanense对非生物压力的反应中发挥着重要作用.
已识别的基因是进一步研究其在Dendrobium中的生物功能的潜在候选者.
提供了对这种物种中CYP450基因的功能动态的基础见解.

关键词:

无生物应激应激是一种非生物应激.细胞染色体P450的使用.丹德罗比亚姆 (Dendrobium huoshanenseense) 是一种植物.表达方式表达模式.基因家族基因家族

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