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Updated: Jul 14, 2026

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
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光传感器用于基于SNAr反应的神经元中氨基酸神经递质的可视化

Ming Xu1, Peeyush Yadav1, Xin Liu2

  • 1Department of Chemistry, University of Missouri, Columbia, Missouri 65211, United States.

ACS chemical neuroscience
|March 11, 2025
PubMed
概括

一个新的基于库马林的光传感器能够在神经元中选择性地可视化像谷氨酸这样的氨基酸. 这一突破使得神经递质活动的图像更清晰,克服了以前的可视化挑战.

关键词:
对于GABA来说,这是一个很好的选择.光成像成像技术的使用.谷氨酸酸盐的使用方法这些神经递质是神经递质.核友芳香替代的核友芳香替代是一种

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...