油通过ROS/NOX4/Smad3轴对 Ferroptosis 进行升级,从而影响人体带网状细胞的纤维化
Jing Wang1, Yang Zhang1, Huimin Zhong2
1Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China.
Investigative ophthalmology & visual science
|March 11, 2025
概括
油 (SiO) 通过损伤状网状细胞,可以引起二次光眼. 这项研究揭示了铁亡和ROS/NOX4/Smad3通路是关键机制,提供了预防并发症的目标.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 在复杂的情况下,油 (SiO) 对于视网膜重新连接至关重要.
- 二次性玻璃眼瘤是内SiO吸管的重要并发症.
- 椎间板状网状细胞 (HTMCs) 对于调节眼内压力至关重要.
研究的目的:
- 为了研究SiO对HTMC的影响.
- 阐明SiO诱导的眼部并发症背后的机制.
- 确定潜在的治疗目标,以减轻与SiO相关的不良影响.
主要方法:
- 在HTMC与SiO的共同培养中.
- 评估细胞增殖,纤维化标志物和铁.
- 基因敲除NOX4和Smad3以探索潜在的途径.
主要成果:
- SiO抑制了HTMC的扩散,并增加了纤维化标志物.
- 在HTMC中,SiO提高了铁化水平.
- 通过NOX4上调和Smad3激活,SiO诱导的铁和ROS增加.
结论:
- 铁和ROS/NOX4/Smad3轴对SiO诱导的眼内压升高至关重要.
- 这些发现为潜在的治疗策略提供了洞察力.
- 缓解与SiO吸管相关的术后并发症是一个关键目标.
相关概念视频
01:26Protein Modifications in the RER
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
01:16Necrosis
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become anucleated and die, but their...