Paenibacillus campi sp. 这种种植物的种类. nov.,一种新型内性细菌,从Pennisetum物种中分离出来
Ling Yao1, Manik Prabhu Narsing Rao2, Cheng-Jie Xie1
1Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China.
概括
两种新的内菌, *Paenibacillus campi* sp. 这两种新型的内菌. 新的菌株SGZ-1009T和SGZ-1014,是从Pennisetum sp.中鉴定出来的 这些细菌表现出独特的生理特征和遗传特征,使它们成为Paenibacillus*属中的新物种.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 植物与微生物的相互作用
背景情况:
- 植物内生细菌居住在植物组织中,在植物健康和发育中起着至关重要的作用.
- *Paenibacillus*属包括多种多样的,通常是有益的,具有重要的生态和工业应用的グラム阳性细菌.
- 对新型内菌的隔离和表征对于了解植物微生物组和发现新的微生物资源至关重要.
研究的目的:
- 从Pennisetum sp.中分离和描述新的内菌.
- 使用多相方法确定孤立菌株SGZ-1009T和SGZ-1014的分类地位.
- 根据表型和基因型数据,在 *Paenibacillus* 属内提出一种新物种.
主要方法:
- 从 *Pennisetum* sp. 中分离了内性细菌. 植物组织.植物组织.
- 生理学特征包括生长条件 (温度,pH,盐度耐受性).
- 基于16S rRNA基因测序和全基因组分析 (ANI,dDDH) 的遗传学分析.
- 化学分类学分析,包括脂肪酸分析,呼吸系统子测定和极性脂质分析.
主要成果:
- 分离了两种选择性有氧,格拉姆阳性,内菌形成的内菌,菌株SGZ-1009T和SGZ-1014.
- 遗传学分析将这些菌株置于*Paenibacillus*属内,与*Paenibacillus wenxiniae*密切相关 (97.0%的16S rRNA基因相似性).
- 基因组相关性 (ANI 81.6%,dDDH 26.0%) 和不同的化学分类学特征 (占主导地位的脂肪酸anteiso-C15:0,MK-7,主要极性脂质DPG,PG,PE) 支持将其指定为新物种.
- 菌株SGZ-1009T表现出广泛的生长范围:550°C,pH4.511.0,高达4.5%的NaCl.
结论:
- 菌株SGZ-1009T和SGZ-1014代表了Paenibacillus*属的一个新物种.
- 这种新物种的建议名称是 *Paenibacillus campi* sp. 在新的一年里.
- 这种类型的菌株是SGZ-1009T (=MCCC 1K08892T=GDMCC 1.4280T=KCTC 43677T=JCM 36670T).
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