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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Updated: May 23, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

在神经干细胞中使用CRISPR技术进行有针对性的人类编辑.

Alvaro Gutierrez-Uzquiza^1,2, Paloma Bragado^3,4

¹Department of Biochemistry and Molecular Biology, Pharmacy Faculty, Complutense University of Madrid, Madrid, Spain. alguuz@ucm.es.

Methods in molecular biology (Clifton, N.J.)

|March 11, 2025

概括

这项研究介绍了哺乳动物细胞中CRISPR-Cas9基因编辑的lentiviral协议. 这种方法通过为研究应用提供精确的DNA修改来促进基因功能的研究.

关键词:

这就是CRISPR/Cas9的作用.基因编辑基因编辑林氏病毒 (Lentivirus) 是一种隐形病毒.神经干细胞的神经干细胞

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Last Updated: May 23, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

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科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物技术是生物技术.

背景情况:

CRISPR-Cas9是一种强大的基因编辑工具,利用指导RNA和Cas9酶来诱导向的DNA双链断裂.
这些断裂是通过非同源端连接 (NHEJ) 修复的,用于突变或同源导向修复 (HDR) 进行精确的编辑.
对成功的基因组编辑应用来说,有效地传递CRISPR组件至关重要,包括生成淘汰模式.

研究的目的:

为哺乳动物细胞中CRISPR-Cas9基因编辑提供一个强大的协议.
为了有效地提供CRISPR-Cas9系统,利用lentiviral载体.
通过精确的基因组修改,促进对基因功能的研究.

主要方法:

开发一种基于lentiviral的传递系统,用于CRISPR-Cas9组件.
该协议应用于用于基因编辑的哺乳动物细胞系.
使用导向RNA将Cas9核酶活动引导到特定的基因组位置.

主要成果:

在哺乳动物细胞中通过CRISPR-Cas9.9成功生成向基因编辑.
作为CRISPR组件在各种细胞类型中的有效输送方法,表现了晶状病毒载体.
通过可靠的基因组编辑促进基因功能研究.

结论:

晶状病毒输送协议为哺乳动物细胞中CRISPR-Cas9介导的基因组编辑提供了一种有价值的方法.
这种方法增强了在体外和体外模型中对基因功能的研究.
该协议支持基础科学,生物技术和生物医学研究的各种研究应用.